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  • Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-FITC

Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-FITC

Cat. No.:
4058-02
Goat Anti-Rabbit IgG Fab-FITC antibody with minimal reactivity to mouse and human serum proteins for use in fluorescent assays.
$105.00
Size Price (USD) Quantity
0.5 mg $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the Fab region of rabbit IgG
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Rabbit IgG Fc; mouse and human serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG Fab covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895788

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. FLISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-FITC (SB Cat. No. 4041-02).
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  2. Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  4. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-FITC (SB Cat. No. 4054-02).
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  5. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  6. Human fibroblast-like synoviocytes were stained with anti-RelA followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-FITC (SB Cat. No. 4050-02) and DAPI.<br/>Image from Crowley T, O'Neil JD, Adams H, Thomas AM, Filer A, Buckley CD, et al. Priming in response to pro-inflammatory cytokines is a feature of adult synovial but not dermal fibroblasts. Arthritis Res Ther. 2017;19:35. Figure S1<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-FITC (SB Cat. No. 4030-02).
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  9. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-FITC (SB Cat. No. 4010-02).
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  10. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  11. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-FITC (SB Cat. No. 4090-02).
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  12. FLISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-FITC (SB Cat. No. 4020-02).
    Goat Anti-Rabbit IgM-FITC
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  13. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rabbit F(ab')2 IgG-UNLB
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  14. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-FITC (SB Cat. No. 4052-02).
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  15. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L)-FITC (SB Cat. No. 4055-02).
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