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Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-BIOT

Cat. No.:
4058-08
Goat Anti-Rabbit IgG Fab-Biotin antibody with minimal reactivity to mouse and human serum proteins for use in ELISA assays.
$105.00
Size Price (USD) Quantity
0.5 mg $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the Fab region of rabbit IgG
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Rabbit IgG Fc; mouse and human serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG Fab covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895790

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  2. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  3. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgM-BIOT
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  4. Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.<br/>Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca<sup>2+</sup>/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG-BIOT
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  7. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-BIOT (SB Cat. No. 4010-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  8. Rabbit peripheral blood lymphocytes were stained with Rat Anti-Rabbit Ig-BIOT (SB Cat. No. 4065-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  9. Rabbit peripheral blood lymphocytes were stained with Goat Anti-Rabbit IgG(H+L), Human ads-BIOT (SB Cat. No. 4051-08) followed by Streptavidin-FITC (SB Cat. No. 7105-02).
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  10. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  11. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-BIOT (SB Cat. No. 4090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Rabbit IgG-BIOT (2A9)
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  12. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab-BIOT (SB Cat. No. 4040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG Fab-BIOT
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  13. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT
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  14. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L)-BIOT (SB Cat. No. 4055-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05).
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