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  • Goat Anti-Rabbit Ig, Human ads-FITC

Goat Anti-Rabbit Ig, Human ads-FITC

Cat. No.:
4010-02
Goat Anti-Rabbit Ig-FITC antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$72.00
Size Price (USD) Quantity
1.0 mg $72.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the heavy and light chains of rabbit IgG and IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Flow Cytometry 4

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 5-7
Immunohistochemistry-Paraffin Sections 3,8
Immunocytochemistry 9-14
Western Blot 15-25
Immunoprecipitation 26
RRID Number AB_2795915

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Fotopoulou S, Oikonomou N, Grigorieva E, Nikitopoulou I, Paparountas T, Thanassopoulou A, et al. ATX expression and LPA signalling are vital for the development of the nervous system. Dev Biol. 2010;339:451-64. (ELISA)
  2. 2. Jain S, Singh SR, Horn DW, Davis VA, Ram MK, Pillai S. Development of an antibody functionalized carbon nanotube biosensor for foodborne bacterial pathogens. J Biosens Bioelectron. 2012;S11:002. (ELISA)
  3. 3. Bétemps D, Verchère J, Brot S, Morignat E, Bousset L, Gaillard D, et al. Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological α-synuclein by enhanced ELISA. Acta Neuropathol Commun. 2014;2:29. (ELISA, IHC-PS)
  4. 4. Hautefort I, Proença MJ, Hinton JC. Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells. Appl Environ Microbiol. 2003;69:7480-91. (FC)
  5. 5. Perry LL, Feilzer K, Portis JL, Caldwell HD. Distinct homing pathways direct T lymphocytes to the genital and intestinal mucosae in Chlamydia-infected mice. J Immunol. 1998;160:2905-14. (IHC-FS)
  6. 6. Manley HA, Lennon VA. Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells. J Histochem Cytochem. 2001;49:1235-43. (IHC-FS)
  7. 7. Gruver AL, Ventevogel MS, Sempowski GD. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution. J Endocrinol. 2009;203:75-85. (IHC-FS)
  8. 8. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, et al. A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chin Sci Bull. 2013;58:2447-53. (IHC-PS)
  9. 9. Campana D, van Dongen JJ, Mehta A, Coustan-Smith E, Wolvers-Tettero IL, Ganeshaguru K, et al. Stages of T-cell receptor protein expression in T-cell acute lymphoblastic leukemia. Blood. 1991;77:1546-54. (ICC)
  10. 10. Ivan M, Bond JA, Prat M, Comoglio PM, Wynford-Thomas D. Activated ras and ret oncogenes induce over-expression of c-met (hepatocyte growth factor receptor) in human thyroid epithelial cells. Oncogene. 1997;14:2417-23. (ICC)
  11. 11. Alonso M, Tamasdan C, Miller DC, Newcomb EW. Flavopiridol induces apoptosis in glioma cell lines independent of retinoblastoma and p53 tumor suppressor pathway alterations by a caspase-independent pathway. Mol Cancer Ther. 2003;2:139-50. (ICC)
  12. 12. Lechuga CG, Hernández-Nazara ZH, Hernández E, Bustamante M, Desierto G, Cotty A, et al. PI3K is involved in PDGF-β receptor upregulation post-PDGF-BB treatment in mouse HSC. Am J Physiol Gastrointest Liver Physiol. 2006;291:G1051-61. (ICC)
  13. 13. Haegens A, Vernooy JH, Heeringa P, Mossman BT, Wouters EF. Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. Eur Respir J. 2008;31:252-60. (ICC)
  14. 14. Bishop RK, Valle Oseguera CA, Spencer JV. Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells. Cancer Cell Microenviron. 2015;2(1):pii: e678. (ICC)
  15. 15. Webb CF, Yamashita Y, Ayers N, Evetts S, Paulin Y, Conley ME, et al. The transcription factor Bright associates with Bruton's tyrosine kinase, the defective protein in immunodeficiency disease. J Immunol. 2000;165:6956-65. (WB)
  16. 16. Pilling D, Buckley CD, Salmon M, Gomer RH. Inhibition of fibrocyte differentiation by serum amyloid P. J Immunol. 2003;171:5537-46. (WB)
  17. 17. Bai Q, Garver JA, Hukriede NA, Burton EA. Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene. Nucleic Acids Res. 2007;35:6501-16. (WB)
  18. 18. Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (WB)
  19. 19. Palatinus JA, O'Quinn MP, Barker RJ, Harris BS, Jourdan J, Gourdie RG. ZO-1 determines adherens and gap junction localization at intercalated disks. Am J Physiol Heart Circ Physiol. 2011;300:H583-94. (WB)
  20. 20. Sommer A, Christensen E, Schwenger S, Seul R, Haas D, Olbrich H, et al. The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 2011;1812:685-90. (WB)
  21. 21. Babcock AS, Anderson AE, Rice CD. Indirubin-3'-(2,3 dihydroxypropyl)-oximether (E804) is a potent modulator of LPS-stimulated macrophage functions. Toxicol Appl Pharmacol. 2013;266:157-66. (WB)
  22. 22. Chen Z, Jolley B, Caldwell C, Gallie DR. Eukaryotic translation initiation factor eIFiso4G is required to regulate violaxanthin de-epoxidase expression in Arabidopsis. J Biol Chem. 2014;289:13926-36. (WB)
  23. 23. Andersen TR, Schmidt JF, Thomassen M, Hornstrup T, Frandsen U, Randers MB, et al. A preliminary study: effects of football training on glucose control, body composition, and performance in men with type 2 diabetes. Scand J Med Sci Sports. 2014;24 Suppl 1:43-56. (WB)
  24. 24. Palatinus JA, Gourdie RG. Diabetes increases cryoinjury size with associated effects on Cx43 gap junction function and phosphorylation in the mouse heart. J Diabetes Res. 2015;2015:510935. (WB)
  25. 25. Xayarath B, Alonzo F 3rd, Freitag NE. Identification of a peptide-pheromone that enhances Listeria monocytogenes escape from host cell vacuoles. PLoS Pathog. 2015;11(3):e1004707. (WB)
  26. 26. Jurivich DA, Pachetti C, Qiu L, Welk JF. Salicylate triggers heat shock factor differently than heat. J Biol Chem. 1995;270:24489-95. (IP)
See All References

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  2. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-FITC (SB Cat. No. 4054-02).
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  4. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-FITC (SB Cat. No. 6440-02) and DAPI.
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  5. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  6. Human fibroblast-like synoviocytes were stained with anti-RelA followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-FITC (SB Cat. No. 4050-02) and DAPI.<br/>Image from Crowley T, O'Neil JD, Adams H, Thomas AM, Filer A, Buckley CD, et al. Priming in response to pro-inflammatory cytokines is a feature of adult synovial but not dermal fibroblasts. Arthritis Res Ther. 2017;19:35. Figure S1<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. FLISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-FITC (SB Cat. No. 4041-02).
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  10. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  12. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L)-FITC (SB Cat. No. 6441-02).
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  13. FLISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-FITC (SB Cat. No. 4020-02).
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  14. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  15. FLISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-FITC (SB Cat. No. 4052-02).
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