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  • Goat Anti-Rabbit Ig, Human ads-BIOT

Goat Anti-Rabbit Ig, Human ads-BIOT

Cat. No.:
4010-08
Goat Anti-Rabbit Ig-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, and immunoprecipitation assays.
$88.00
Size Price (USD) Quantity
1.0 mg $88.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy and light chains of rabbit IgG and IgM
Source Pooled antisera from goats hyperimmunized with rabbit IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-3
FLISA
Flow Cytometry 4

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 5-7
Immunohistochemistry-Paraffin Sections 3,8
Immunocytochemistry 9-14
Western Blot 15-25
Immunoprecipitation 26
RRID Number AB_2795919

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Fotopoulou S, Oikonomou N, Grigorieva E, Nikitopoulou I, Paparountas T, Thanassopoulou A, et al. ATX expression and LPA signalling are vital for the development of the nervous system. Dev Biol. 2010;339:451-64. (ELISA)
  2. 2. Jain S, Singh SR, Horn DW, Davis VA, Ram MK, Pillai S. Development of an antibody functionalized carbon nanotube biosensor for foodborne bacterial pathogens. J Biosens Bioelectron. 2012;S11:002. (ELISA)
  3. 3. Bétemps D, Verchère J, Brot S, Morignat E, Bousset L, Gaillard D, et al. Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological α-synuclein by enhanced ELISA. Acta Neuropathol Commun. 2014;2:29. (ELISA, IHC-PS)
  4. 4. Hautefort I, Proença MJ, Hinton JC. Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells. Appl Environ Microbiol. 2003;69:7480-91. (FC)
  5. 5. Perry LL, Feilzer K, Portis JL, Caldwell HD. Distinct homing pathways direct T lymphocytes to the genital and intestinal mucosae in Chlamydia-infected mice. J Immunol. 1998;160:2905-14. (IHC-FS)
  6. 6. Manley HA, Lennon VA. Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells. J Histochem Cytochem. 2001;49:1235-43. (IHC-FS)
  7. 7. Gruver AL, Ventevogel MS, Sempowski GD. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution. J Endocrinol. 2009;203:75-85. (IHC-FS)
  8. 8. Sang Y, Zhou T, Li H, Ran Y, Jiang D, Zheng F, et al. A new long-acting GLP-1 derivative KTP ameliorates hyperglycemia and dyslipidemia and improves pancreas and fatty liver in db/db mice. Chin Sci Bull. 2013;58:2447-53. (IHC-PS)
  9. 9. Campana D, van Dongen JJ, Mehta A, Coustan-Smith E, Wolvers-Tettero IL, Ganeshaguru K, et al. Stages of T-cell receptor protein expression in T-cell acute lymphoblastic leukemia. Blood. 1991;77:1546-54. (ICC)
  10. 10. Ivan M, Bond JA, Prat M, Comoglio PM, Wynford-Thomas D. Activated ras and ret oncogenes induce over-expression of c-met (hepatocyte growth factor receptor) in human thyroid epithelial cells. Oncogene. 1997;14:2417-23. (ICC)
  11. 11. Alonso M, Tamasdan C, Miller DC, Newcomb EW. Flavopiridol induces apoptosis in glioma cell lines independent of retinoblastoma and p53 tumor suppressor pathway alterations by a caspase-independent pathway. Mol Cancer Ther. 2003;2:139-50. (ICC)
  12. 12. Lechuga CG, Hernández-Nazara ZH, Hernández E, Bustamante M, Desierto G, Cotty A, et al. PI3K is involved in PDGF-β receptor upregulation post-PDGF-BB treatment in mouse HSC. Am J Physiol Gastrointest Liver Physiol. 2006;291:G1051-61. (ICC)
  13. 13. Haegens A, Vernooy JH, Heeringa P, Mossman BT, Wouters EF. Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents. Eur Respir J. 2008;31:252-60. (ICC)
  14. 14. Bishop RK, Valle Oseguera CA, Spencer JV. Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells. Cancer Cell Microenviron. 2015;2(1):pii: e678. (ICC)
  15. 15. Webb CF, Yamashita Y, Ayers N, Evetts S, Paulin Y, Conley ME, et al. The transcription factor Bright associates with Bruton's tyrosine kinase, the defective protein in immunodeficiency disease. J Immunol. 2000;165:6956-65. (WB)
  16. 16. Pilling D, Buckley CD, Salmon M, Gomer RH. Inhibition of fibrocyte differentiation by serum amyloid P. J Immunol. 2003;171:5537-46. (WB)
  17. 17. Bai Q, Garver JA, Hukriede NA, Burton EA. Generation of a transgenic zebrafish model of Tauopathy using a novel promoter element derived from the zebrafish eno2 gene. Nucleic Acids Res. 2007;35:6501-16. (WB)
  18. 18. Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (WB)
  19. 19. Palatinus JA, O'Quinn MP, Barker RJ, Harris BS, Jourdan J, Gourdie RG. ZO-1 determines adherens and gap junction localization at intercalated disks. Am J Physiol Heart Circ Physiol. 2011;300:H583-94. (WB)
  20. 20. Sommer A, Christensen E, Schwenger S, Seul R, Haas D, Olbrich H, et al. The molecular basis of aminoacylase 1 deficiency. Biochim Biophys Acta. 2011;1812:685-90. (WB)
  21. 21. Babcock AS, Anderson AE, Rice CD. Indirubin-3'-(2,3 dihydroxypropyl)-oximether (E804) is a potent modulator of LPS-stimulated macrophage functions. Toxicol Appl Pharmacol. 2013;266:157-66. (WB)
  22. 22. Chen Z, Jolley B, Caldwell C, Gallie DR. Eukaryotic translation initiation factor eIFiso4G is required to regulate violaxanthin de-epoxidase expression in Arabidopsis. J Biol Chem. 2014;289:13926-36. (WB)
  23. 23. Andersen TR, Schmidt JF, Thomassen M, Hornstrup T, Frandsen U, Randers MB, et al. A preliminary study: effects of football training on glucose control, body composition, and performance in men with type 2 diabetes. Scand J Med Sci Sports. 2014;24 Suppl 1:43-56. (WB)
  24. 24. Palatinus JA, Gourdie RG. Diabetes increases cryoinjury size with associated effects on Cx43 gap junction function and phosphorylation in the mouse heart. J Diabetes Res. 2015;2015:510935. (WB)
  25. 25. Xayarath B, Alonzo F 3rd, Freitag NE. Identification of a peptide-pheromone that enhances Listeria monocytogenes escape from host cell vacuoles. PLoS Pathog. 2015;11(3):e1004707. (WB)
  26. 26. Jurivich DA, Pachetti C, Qiu L, Welk JF. Salicylate triggers heat shock factor differently than heat. J Biol Chem. 1995;270:24489-95. (IP)
See All References

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