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Goat Anti-Mouse Lambda-HRP

Goat Anti-Mouse Lambda-HRP

Name: Goat Anti-Mouse Lambda-HRP
SKU: 1060-05
Price: 193 USD
Availability: InStock

Cat. No.:

1060-05

Goat Anti-Mouse Lambda-HRP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays.

$193.00

Size	Price (USD)	Quantity
1.0 mL	$193.00

Qty

Technical Bulletin Safety Datasheet

Lane 1 - Mouse IgG<sub>1</sub>κ<br/>Lane 2 - Mouse IgG<sub>2a</sub>λ<br/>Lane 3 - Mouse IgG<sub>2b</sub>κ<br/>Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-M

Lane 1 - Mouse IgG₁κ
Lane 2 - Mouse IgG_2aλ
Lane 3 - Mouse IgG_2bκ
Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-M

ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-HRP (SB Cat. No. 1060-05).

Product Specifications

More Information
Isotype	Goat IgG
Specificity	Reacts with mouse λ light chains
Source	Pooled antisera from goats hyperimmunized with mouse λ light chains
Cross Adsorption	Mouse κ light chains; may react with λ light chains from other species
Purification Method	Affinity chromatography on mouse λ light chains covalently linked to agarose
Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality	Polyclonal
Concentration	Lot specific
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-6,23 FLISA Flow Cytometry ^3,5,7-13,24 Other referenced applications for relevant formats include - ELISpot ^6,7 Immunohistochemistry-Frozen Sections ^2,13-16 Immunohistochemistry-Paraffin Sections ^17,23 Immunocytochemistry ^1,4,18 Western Blot ^19,20,25 Immunoprecipitation ^21,22 Stimulation ^23,24 Separation ²⁵
RRID Number	AB_2794393
Gene ID	111519 (Mouse)
Gene ID Symbol	Igl (Mouse)
Gene ID Aliases	Igl-x

Documentation

Technical Bulletin Safety Datasheet

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Other Conjugates and Formulations

Citations

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16. Rouaud P, Saintamand A, Saad F, Carrion C, Lecardeur S, Cogné M, et al. Elucidation of the enigmatic IgD class-switch recombination via germline deletion of the IgH 3' regulatory region. J Exp Med. 2014;211:975-85. (IHC-FS)
17. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunol. 2008;181:3259-67. (IHC-PS)
18. Ghumra A, Shi J, Mcintosh RS, Rasmussen IB, Braathen R, Johansen F, et al. Structural requirements for the interaction of human IgM and IgA with the human Fcα/μ receptor. Eur J Immunol. 2009;39:1147-56. (ICC)
19. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in C_H1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
20. Sato M, Adachi T, Tsubata T. Augmentation of signaling through BCR containing IgE but not that containing IgA due to lack of CD22-mediated signal regulation. J Immunol. 2007;178:2901-7. (WB)
21. Shen Y, Meunier L, Hendershot LM. Identification and characterization of a novel endoplasmic reticulum (ER) DnaJ homologue, which stimulates ATPase activity of BiP in vitro and is induced by ER stress. J Biol Chem. 2002;277:15947-56. (IP)
22. Behnke J, Hendershot LM. The large Hsp70 Grp170 binds to unfolded protein substrates in vivo with a regulation distinct from conventional Hsp70s. J Biol Chem. 2014;289:2899-907. (IP)
23. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (ELISA, IHC-FS, Stim)
24. Übelhart R, Bach MP, Eschbach C, Wossning T, Reth M, Jumaa H. N-linked glycosylation selectively regulates autonomous precursor BCR function. Nat Immunol. 2010;11:759-65. (FC, Stim)
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