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Goat Anti-Mouse Kappa-PE
Cat. No.:
1050-09
Goat Anti-Mouse Kappa-PE antibody for use in flow cytometry assays.
$297.00
| Isotype | Goat IgG |
|---|---|
| Isotype Control | Goat IgG-PE |
| Specificity | Reacts with mouse κ light chains |
| Source | Pooled antisera from goats hyperimmunized with mouse κ light chains |
| Cross Adsorption | Mouse λ light chains; may react with κ light chains from other species |
| Purification Method | Affinity chromatography on mouse κ light chains covalently linked to agarose |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications |
Quality tested applications for relevant formats include - ELISA 1-11 FLISA Flow Cytometry 3,8,12,15,16,26 Other referenced applications for relevant formats include - ELISpot 2,7,12-14 Immunohistochemistry-Frozen Sections 17 Immunohistochemistry-Paraffin Sections 18,19 Immunocytochemistry 5,20-22 Western Blot 1,4,6,8,9,15,26 Immunoprecipitation 23,24 Stimulation 25,26 Surface Plasmon Resonance 27 |
| RRID Number | AB_2794384 |
| Gene ID |
243469 (Mouse) |
| Gene ID Symbol |
Igk (Mouse) |
| Gene ID Aliases | kappa |
| UniProt ID |
P01837 (Mouse |
| UniProt Name |
IGKC_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. Hemminki A, Niemi S, Hoffrén A, Hakalahti L, Söderlund H, Takkinen K. Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection. Protein Eng. 1998;11:311-9. (ELISA, WB)
- 2. Sangster MY, Topham DJ, D'Costa S, Cardin RD, Marion TN, Myers LK, et al. Analysis of the virus-specific and nonspecific B cell response to a persistent B-lymphotropic gammaherpesvirus. J Immunol. 2000;164:1820-8. (ELISA, ELISPOT)
- 3. D'Agnillo P, Levine JS, Subang R, Rauch J. Prothrombin binds to the surface of apoptotic, but not viable, cells and serves as a target of lupus anticoagulant autoantibodies. J Immunol. 2003;170:3408-22. (ELISA, FC)
- 4. Wiens GD, Owen J. Mapping of neutralizing epitopes on Renibacterium salmoninarum p57 by use of transposon mutagenesis and synthetic peptides. Appl Environ Microbiol. 2005;71:2894-901. (ELISA, WB)
- 5. Chen C, Li H, Tian Q, Beardall M, Xu Y, Casanova N, et al. Selection of anti-double-stranded DNA B cells in autoimmune MRL-lpr/lpr mice. J Immunol. 2006;176:5183-90. (ELISA, ICC)
- 6. Subhadra B, Hurwitz I, Fieck A, Rao DV, Rao GS, Durvasula R. Development of paratransgenic Artemia as a platform for control of infectious diseases in shrimp mariculture. J Appl Microbiol. 2010;108:831-40. (ELISA, WB)
- 7. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Iga serine/threonines fine-tune Ig a tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
- 8. Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (ELISA, FC, WB)
- 9. Nakanishi K, Narimatsu S, Ichikawa S, Tobisawa Y, Kurohane K, Niwa Y, et al. Production of hybrid-IgG/IgA plantibodies with neutralizing activity against Shiga toxin 1. PLoS One. 2013;8(11):e80712. (ELISA, WB)
- 10. DiLillo DJ, Tan GS, Palese P, Ravetch JV. Broadly neutralizing hemagglutinin stalk-specific antibodies require FcγR interactions for protection against influenza virus in vivo. Nat Med. 2014;20:143-51. (ELISA)
- 11. Sang A, Niu H, Cullen J, Choi SC, Zheng YY, Wang H, et al. Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. J Immunol. 2014;193:1609-21. (ELISA)
- 12. Seagal J, Edry E, Keren Z, Leider N, Benny O, Machluf M, et al. A fail-safe mechanism for negative selection of isotype-switched B cell precursors is regulated by the Fas/FasL pathway. J Exp Med. 2003;198:1609-19. (ELISPOT, FC)
- 13. Sharma S, Campbell AM, Chan J, Schattgen SA, Orlowski GM, Nayar R, et al. Suppression of systemic autoimmunity by the innate immune adaptor STING. Proc Natl Acad Sci USA. 2015;112:E710-7. (ELISPOT)
- 14. Ratliff M, Alter S, McAvoy K, Frasca D, Wright JA, Zinkel SS, et al. In aged mice, low surrogate light chain promotes pro-B-cell apoptotic resistance, compromises the PreBCR checkpoint, and favors generation of autoreactive, phosphorylcholine-specific B cells. Aging Cell. 2015;14:382-90. (ELISPOT)
- 15. Rakhmanov M. Analysis of B lymphocyte activation and differentiation by expression profiling [dissertation]. Freiburg (Germany); University of Freiburg: 2005. (FC, WB)
- 16. Campbell KS, Yusa S, Kikuchi-Maki A, Catina TL. NKp44 triggers NK cell activation through DAP12 association that is not influenced by a putative cytoplasmic inhibitory sequence. J Immunol. 2004;172:899-906. (FC)
- 17. Harris DP, Vogel P, Wims M, Moberg K, Humphries J, Jhaver KG, et al. Requirement for class II phosphoinositide 3-kinase C2α in maintenance of glomerular structure and function. Mol Cell Biol. 2011;31:63-80. (IHC-FS)
- 18. Verdonk RC, Haagsma EB, Jonker MR, Bok LI, Zandvoort JH, Kleibeuker JH, et al. Effects of different immunosuppressive regimens on regulatory T-cells in noninflamed colon of liver transplant recipients. Inflamm Bowel Dis. 2007;13:703-9. (IHC-PS)
- 19. Weinstein JS, Nacionales DC, Lee PY, Kelly-Scumpia KM, Yan X, Scumpia PO, et al. Colocalization of antigen-specific B and T cells within ectopic lymphoid tissue following immunization with exogenous antigen. J Immunmol. 2008;181:3259-67. (IHC-PS)
- 20. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig λ-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ICC)
- 21. Duchez S, Amin R, Cogné N, Delpy L, Sirac C, Pascal V, et al. Premature replacement of μ with α immunoglobulin chains impairs lymphopoiesis and mucosal homing but promotes plasma cell maturation. Proc Natl Acad Sci USA. 2010;107:3064-9. (ICC)
- 22. Wang N, Yuan A, Ma J, Deng Z, Xue L. Sexing murine embryos with an indirect immunofluorescence assay using phage antibody B9-Fab against SDM antigen. J Vet Med Sci. 2015;77:711-4. (ICC)
- 23. Skowronek MH, Hendershot LM, Haas IG. The variable domain of nonassembled Ig light chains determines both their half-life and binding to the chaperone BiP. Proc Natl Acad Sci USA. 1998;95:1574-8. (IP)
- 24. Otero JH, Lizák B, Feige MJ, Hendershot LM. Dissection of structural and functional requirements that underlie the interaction of ERdj3 protein with substrates in the endoplasmic reticulum. J Biol Chem. 2014;289:27504-12. (IP)
- 25. Ku P, You M, Bose HR Jr. Role and regulation of Rel/NF-κB activity in anti-immunoglobulin-induced apoptosis in WEHI-231 B lymphoma cells. Cell Signal. 2000;12:245-53. (Stim)
- 26. Übelhart R, Hug E, Bach MP, Wossning T, Dühren-von Minden M, Horn AH, et al. Responsiveness of B cells is regulated by the hinge region of IgD. Nat Immunol. 2015;16:534-43. (FC, WB, Stim)
- 27. Swearingen CA, Carpenter JW, Siegel R, Brittain IJ, Dotzlaf J, Durham TB, et al. Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine. Osteoarthritis Cartilage. 2010;18:1150-8. (Surface Plasmon Resonance)
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