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Goat Anti-Mouse IgM, Human ads-UNLB

Goat Anti-Mouse IgM, Human ads-UNLB

Name: Goat Anti-Mouse IgM, Human ads-UNLB
SKU: 1020-01
Price: 127 USD
Availability: InStock

Cat. No.:

1020-01

Purified Goat Anti-Mouse IgM antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blot, immunoprecipitation, ELISpot, purification, and stimulation assays.

$127.00

Size	Price (USD)	Quantity
1.0 mg	$127.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified mouse IgM, IgG, and IgA and human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 61

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-UNLB followed by Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01) and Mouse Anti-Goat IgG Fc-AF488 (SB Cat. No. 6158-30).

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	Goat IgG-UNLB
Specificity	Reacts with the heavy chain of mouse IgM
Source	Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption	Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on mouse IgM covalently linked to agarose
Conjugate	UNLB (Unconjugated)
Buffer Formulation	Borate buffered saline, pH 8.2
Clonality	Polyclonal
Concentration	1.0 mg/mL
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-11 FLISA Flow Cytometry ^4,7,10,13-17 Other referenced applications for relevant formats include - ELISpot ^{2,3,5,6,8-10,12} Immunohistochemistry-Frozen Sections ^1,7,18-22 Immunohistochemistry-Paraffin Sections ²⁸ Immunocytochemistry ^1,13,23 Electron Microscopy ¹⁹ Western Blot ^14,16,24 Immunoprecipitation ^24,29,30 Purification ²⁶ Stimulation ^14,25-27
RRID Number	AB_2794197
Gene ID	16019 (Mouse)
Gene ID Symbol	Ighm (Mouse)
Gene ID Aliases	AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID	P01872 (Mouse
UniProt Name	IGHM_MOUSE (Mouse)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

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9. Goenka R, Matthews AH, Zhang B, O'Neill PJ, Scholz JL, Migone T, et al. Local BLyS production by T follicular cells mediates retention of high affinity B cells during affinity maturation. J Exp Med. 2014;211:45-56. (ELISA, ELISPOT)
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11. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
12. Shin C, Han J, Koh H, Choi B, Cho Y, Jeong H, et al. CD8α^- dendritic cells induce antigen-specific T follicular helper cells generating efficient humoral immune responses. Cell Rep. 2015;11:1929-40. (ELISPOT)
13. Eaton-Bassiri AS, Mandik-Nayak L, Seo S, Madaio MP, Cancro MP, Erikson J. Alterations in splenic architecture and the localization of anti-double-stranded DNA B cells in aged mice. Int Immunol. 2000;12:915-26. (FC, ICC)
14. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (FC, WB, Stim)
15. Zhou X, Xiang Y, Ding X, Garrard WT. A new hypersensitive site, HS10, and the enhancers, E3' and Ed, differentially regulate Igκ gene expression. J Immunol. 2012;188:2722-32. (FC)
16. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC, WB)
17. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
18. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
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20. Neese W, Westra J, van der Wal JE, Abbas F, Nicholas AP, Vissink A, et al. The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation. J Clin Periodontol. 2012;39:599-607. (IHC-FS)
21. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
22. Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. (IHC-FS)
23. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
24. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB, IP)
25. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
26. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (Purification, Stim)
27. Wolkerstorfer S, Schwaiger E, Rinnerthaler M, Gratz IK, Zoegg T, Brandstetter H, et al. HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis. Cell Mol Immunol. 2015;Apr 13:doi: 10.1038/cmi.2015.018. [Epub ahead of print]. (Stim)
28. SouthernBiotech published data (IHC-PS)
29. Koenig P, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, et al. The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice. J BIol Chem. 2014;289:34490-502. (IP)
30. Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J Immunol. 2015;194:3096-101. (IP)