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Goat Anti-Mouse IgM, Human ads-BIOT

Goat Anti-Mouse IgM, Human ads-BIOT

Name: Goat Anti-Mouse IgM, Human ads-BIOT
SKU: 1020-08
Price: 149 USD
Availability: InStock

Cat. No.:

1020-08

Goat Anti-Mouse IgM-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blot, immunoprecipitation, and ELISpot assays.

$149.00

Size	Price (USD)	Quantity
1.0 mg	$149.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).

Paraffin embedded mouse lymph node section was stained with Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02) and DAPI.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-UNLB followed by Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) and Streptavidin-FITC (SB Cat. No. 7100-02).

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	Goat IgG-BIOT
Specificity	Reacts with the heavy chain of mouse IgM
Source	Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption	Mouse IgG and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on mouse IgM covalently linked to agarose
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Polyclonal
Concentration	0.5 mg/mL
Volume	2.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-11 FLISA Flow Cytometry ^4,7,10,13-17 Other referenced applications for relevant formats include - ELISpot ^{2,3,5,6,8-10,12} Immunohistochemistry-Frozen Sections ^1,7,18-22 Immunohistochemistry-Paraffin Sections ²⁸ Immunocytochemistry ^1,13,23 Electron Microscopy ¹⁹ Western Blot ^14,16,24 Immunoprecipitation ^24,29,30 Purification ²⁶ Stimulation ^14,25-27
RRID Number	AB_2737411
Gene ID	16019 (Mouse)
Gene ID Symbol	Ighm (Mouse)
Gene ID Aliases	AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID	P01872 (Mouse
UniProt Name	IGHM_MOUSE (Mouse)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

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9. Goenka R, Matthews AH, Zhang B, O'Neill PJ, Scholz JL, Migone T, et al. Local BLyS production by T follicular cells mediates retention of high affinity B cells during affinity maturation. J Exp Med. 2014;211:45-56. (ELISA, ELISPOT)
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11. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
12. Shin C, Han J, Koh H, Choi B, Cho Y, Jeong H, et al. CD8α^- dendritic cells induce antigen-specific T follicular helper cells generating efficient humoral immune responses. Cell Rep. 2015;11:1929-40. (ELISPOT)
13. Eaton-Bassiri AS, Mandik-Nayak L, Seo S, Madaio MP, Cancro MP, Erikson J. Alterations in splenic architecture and the localization of anti-double-stranded DNA B cells in aged mice. Int Immunol. 2000;12:915-26. (FC, ICC)
14. Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck H, Wabl M, et al. Ig heavy chain promotes mature B cell survival in the absence of light chain. J Immunol. 2007;179:1659-68. (FC, WB, Stim)
15. Zhou X, Xiang Y, Ding X, Garrard WT. A new hypersensitive site, HS10, and the enhancers, E3' and Ed, differentially regulate Igκ gene expression. J Immunol. 2012;188:2722-32. (FC)
16. Alkhatib A, Werner M, Hug E, Herzog S, Eschbach C, Faraidun H, et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med. 2012;209:395-406. (FC, WB)
17. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
18. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
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20. Neese W, Westra J, van der Wal JE, Abbas F, Nicholas AP, Vissink A, et al. The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation. J Clin Periodontol. 2012;39:599-607. (IHC-FS)
21. Simonetti G, Carette A, Silva K, Wang H, De Silva NS, Heise N, et al. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression and activity. J Exp Med. 2013;210:2887-902. (IHC-FS)
22. Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. (IHC-FS)
23. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
24. Elkabetz Y, Shapira I, Rabinovich E, Bar-Nun S. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. J Biol Chem. 2004;279:3980-89. (WB, IP)
25. Kato I, Takai T, Kudo A. The pre-B cell receptor signaling for apoptosis is negatively regulated by FcγRIIB. J Immunol. 2002;168:629-34. (Stim)
26. Su Y, Flemming A, Wossning T, Hobeika E, Reth M, Jumaa H. Identification of a pre-BCR lacking surrogate light chain. J Exp Med. 2003;198:1699-706. (Purification, Stim)
27. Wolkerstorfer S, Schwaiger E, Rinnerthaler M, Gratz IK, Zoegg T, Brandstetter H, et al. HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis. Cell Mol Immunol. 2015;Apr 13:doi: 10.1038/cmi.2015.018. [Epub ahead of print]. (Stim)
28. SouthernBiotech published data (IHC-PS)
29. Koenig P, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, et al. The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice. J BIol Chem. 2014;289:34490-502. (IP)
30. Ouchida R, Lu Q, Liu J, Li Y, Chu Y, Tsubata T, et al. FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J Immunol. 2015;194:3096-101. (IP)