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  • Goat Anti-Mouse IgM-AF647

Goat Anti-Mouse IgM-AF647

Cat. No.:
1021-31
Goat Anti-Mouse IgM-Alexa Fluor® 647 antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$150.00
Size Price (USD) Quantity
1.0 mg $150.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF647
Specificity Reacts with the heavy chain of mouse IgM
Source Pooled antisera from goats hyperimmunized with mouse IgM
Cross Adsorption Mouse IgG and IgA; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgM covalently linked to agarose
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-6
FLISA
Flow Cytometry 4,7-17

Other referenced applications for relevant formats include -
ELISpot 6
Immunohistochemistry-Frozen Sections 18,19
Immunohistochemistry-Paraffin Sections 20
Immunocytochemistry 21,22
Western Blot 23,24
Stimulation 25,26
RRID Number AB_2794254
Gene ID 16019 (Mouse)
Gene ID Symbol Ighm (Mouse)
Gene ID Aliases AI326478; Igh-6; Igh-M; Igh6; Igm; muH
UniProt ID P01872 (Mouse
UniProt Name IGHM_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Gass JN, Gifford NM, Brewer JW. Activation of an unfolded protein response during differentiation of antibody-secreting B cells. J Biol Chem. 2002;277:49047-54. (ELISA)
  2. 2. Chang P, Barral P, Fitch J, Pratama A, Ma CS, Kallies A, et al. Identification of Bcl-6-dependent follicular helper NKT cells that provide cognate help for B cell responses. Nat Immunol. 2012;13:35-43. (ELISA)
  3. 3. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (ELISA)
  4. 4. Teague H, Harris M, Fenton J, Lallemand P, Shewchuk BM, Shaikh SR. Eicosapentaenoic and docosahexaenoic acid ethyl esters differentially enhance B-cell activity in murine obesity. J Lipid Res. 2014;55:1420-33. (ELISA, FC)
  5. 5. Merlo LM, Pigott E, DuHadaway JB, Grabler S, Metz R, Prendergast GC, et al. IDO2 is a critical mediator of autoantibody production and inflammatory pathogenesis in a mouse model of autoimmune arthritis. J Immunol. 2014;192:2082-90. (ELISA)
  6. 6. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA, ELISPOT)
  7. 7. Chen C, Yull FE, Cardwell N, Singh N, Strayhorn WD, Nanney LB, et al. RAG2-/-, IκB-α-/- chimeras display a psoriasiform skin disease. J Invest Dermatol. 2000;115:1124-33. (FC)
  8. 8. Chen C, Singh N, Yull FE, Strayhorn D, Van Kaer L, Kerr LD. Lymphocytes lacking IκB-α develop normally, but have selective defects in proliferation and function. J Immunol. 2000;165:5418-27. (FC)
  9. 9. Erlandsson MC, Jonsson CA, Lindberg MK, Ohlsson C, Carlsten H. Raloxifene- and estradiol-mediated effects on uterus, bone and B lymphocytes in mice. J Endocrinol. 2002;175:319-27. (FC)
  10. 10. Islander U, Erlandsson MC, Hasséus B, Jonsson CA, Ohlsson C, Gustafsson J, et al. Influence of oestrogen receptor α and β on the immune system in aged female mice. Immunology. 2003;110:149-57. (FC)
  11. 11. Erlandsson MC, Jonsson CA, Islander U, Ohlsson C, Carlsten H. Oestrogen receptor specificity in oestradiol-mediated effects on B lymphopoiesis and immunoglobulin production in male mice. Immunology. 2003;108:346-51. (FC)
  12. 12. Bahjat FR, Pine PR, Reitsma A, Cassafer G, Baluom M, Grillo S, et al. An orally bioavailable spleen tyrosine kinase inhibitor delays disease progression and prolongs survival in murine lupus. Arthritis Rheum. 2008;58:1433-44. (FC)
  13. 13. Windahl SH, Andersson N, Chagin AS, MÃ¥rtensson UE, Carlsten H, Olde B, et al. The role of the G protein-coupled receptor GPR30 in the effects of estrogen in ovariectomized mice. Am J Physiol Endocrinol Metab. 2009;296:E490-6. (FC)
  14. 14. Giuriato S, Foisseau M, Dejean E, Felsher DW, Al Saati T, Demur C, et al. Conditional TPM3-ALK and NPM-ALK transgenic mice develop reversible ALK-positive early B-cell lymphoma/leukemia. Blood. 2010;115:4061-70. (FC)
  15. 15. Verma-Gaur J, Hauser J, Grundström T. Negative feedback regulation of antigen receptors through calmodulin inhibition of E2A. J Immunol. 2012;188:6175-83. (FC)
  16. 16. Chen J, Yin H, Xu J, Wang Q, Edelblum KL, Sciammas R, et al. Reversing endogenous alloreactive B cell GC responses with anti-CD154 or CTLA-4Ig. Am J Transplant. 2013;13:2280-92. (FC)
  17. 17. Brady AM, Calix JJ, Yu J, Geno KA, Cutter GR, Nahm MH. Low invasiveness of pneumococcal serotype 11A is linked to ficolin-2 recognition of O-acetylated capsule epitopes and lectin complement pathway activation. J Infect Dis. 2014;210:1155-65. (FC)
  18. 18. DeAngelis RA, Markiewski MM, Kourtzelis I, Rafail S, Syriga M, Sandor A, et al. A complement-IL-4 regulatory circuit controls liver regeneration. J Immunol. 2012;188:641-8. (IHC-FS)
  19. 19. Dubois V, Laurent MR, Sinnesael M, Cielen N, Helsen C, Clinckemalie L, et al. A satellite cell-specific knockout of the androgen receptor reveals myostatin as a direct androgen target in skeletal muscle. FASEB J. 2014;28:2979-94. (IHC-FS)
  20. 20. Pamuk ON, Lapchak PH, Rani P, Pine P, Dalle Lucca JJ, Tsokos GC. Spleen tyrosine kinase inhibition prevents tissue damage after ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol. 2010;299:G391-9. (IHC-PS)
  21. 21. Gills JJ, Zhang C, Abu-Asab MS, Castillo SS, Marceau C, LoPiccolo J, et al. Ceramide mediates nanovesicle shedding and cell death in response to phosphatidylinositol ether lipid analogs and perifosine. Cell Death Differ. 2012;3:e340. (ICC)
  22. 22. Lilja HE, Morrison WA, Han X, Palmer J, Taylor C, Tee R, et al. An adipoinductive role of inflammation in adipose tissue engineering: key factors in the early development of engineered soft tissues. Stem Cells Dev. 2013;22:1602-13. (ICC)
  23. 23. Bergrin M, Bicer S, Lucas CA, Reiser PJ. Three-dimensional compartmentalization of myosin heavy chain and myosin light chain isoforms in dog thyroarytenoid muscle. Am J Physiol Cell Physiol. 2006;290:C1446-58. (WB)
  24. 24. Kuge H, Akahori K, Yagyu K, Honke K. Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids. J Biol Chem. 2014;289:26783-93. (WB)
  25. 25. Donjerković D, Mueller CM, Scott DW. Steroid- and retinoid-mediated growth arrest and apoptosis in WEHI-231 cells: role of NF-κB, c-Myc and CKI p27Kip1. Eur J Immunol. 2000;30:1154-61. (Stim)
  26. 26. Ãœbelhart R, Bach MP, Eschbach C, Wossning T, Reth M, Jumaa H. N-linked glycosylation selectively regulates autonomous precursor BCR function. Nat Immunol. 2010;11:759-65. (Stim)
See All References

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  1. FLISA plate was coated with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-UNLB (SB Cat. No. 6300-01). Serially diluted Goat IgG-AF647 (SB Cat. No. 0109-31) was captured and fluorescence intensity quantified.
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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