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Goat Anti-Mouse IgG3, Human ads-SPRD
Cat. No.:
1100-13
Goat Anti-Mouse IgG3-PE/CY5 (SPRD) antibody with minimal reactivity to human proteins for use in flow cytometry assays.
$270.00
| Isotype | Goat IgG |
|---|---|
| Isotype Control | Goat IgG-SPRD |
| Specificity | Reacts with the heavy chain of mouse IgG3 |
| Source | Pooled antisera from goats hyperimmunized with mouse IgG3 |
| Cross Adsorption | Mouse IgG1, IgG2a, IgG2b, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species |
| Purification Method | Affinity chromatography on mouse IgG3 covalently linked to agarose |
| Conjugate | SPRD (R-phycoerythrin/Cyanine 5) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Polyclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.5 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Trademark Information | Spectral Red® is a registered trademark of Southern Biotechnology Associates, Inc. Spectral Red® is a PE/CY5 tandem conjugate Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH |
| Applications | Quality tested applications for relevant formats include - ELISA 1-9 FLISA 2 Flow Cytometry 10-16 Other referenced applications for relevant formats include - ELISpot 1,3,4,10 Immunohistochemistry-Frozen Sections 17-21 Immunohistochemistry-Paraffin Sections 17,22 Immunocytochemistry 23 Western Blot 1,24 |
| RRID Number | AB_2794578 |
| Gene ID | 380795 (Mouse) |
| Gene ID Symbol | Ighg3 (Mouse) |
| Gene ID Aliases | IgG3 |
| UniProt ID | P03987 (Mouse |
| UniProt Name | IGHG3_MOUSE (Mouse) |
Documentation
Certificate of Analysis Lookup
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- 1. McLay J, Leonard E, Petersen S, Shapiro D, Greenspan NS, Schreiber JR. γ3 gene-disrupted mice selectively deficient in the dominant IgG subclass made to bacterial polysaccharides. II. Increased susceptibility to fatal pneumococcal sepsis due to absence of anti-polysaccharide IgG3 is corrected by induction of anti-polysaccharide IgG1. J Immunol. 2002;168:3437-43. (ELISA, ELISPOT, WB)
- 2. Szmacinski H, Smith DS, Hanson MA, Kostov Y, Lakowicz JR, Rao G. A novel method for monitoring monoclonal antibody production during cell culture. Biotechnol Bioeng. 2008;100:448-57. (ELISA, FLISA)
- 3. Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (ELISA, ELISPOT)
- 4. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
- 5. Förster M, Raposo B, Ekman D, Klaczkowska D, Popovic M, Nandakumar KS, et al. Genetic control of antibody production during collagen-induced arthritis development in heterogeneous stock mice. Arthritis Rheum. 2012;64:3594-603. (ELISA)
- 6. Li X, Wu J, Ptacek T, Redden DT, Brown EE, Alarcón GS, et al. Allelic-dependent expression of an activating Fc receptor on B cells enhances humoral immune responses. Sci Transl Med. 2013;5(216):216ra175. (ELISA)
- 7. Brachs S, Turqueti-Neves A, Stein M, Reimer D, Brachvogel B, Bösl M, et al. Swiprosin-1/EFhd2 limits germinal center responses and humoral type 2 immunity. Eur J Immunol. 2014;44:3206-19. (ELISA)
- 8. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
- 9. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISA)
- 10. Conway KL, Kuballa P, Khor B, Zhang M, Shi HN, Virgin HW, et al. ATG5 regulates plasma cell differentiation. Autophagy. 2013;9:528-37. (ELISPOT, FC)
- 11. Cook AJ, Oganesian L, Harumal P, Basten A, Brink R, Jolly CJ. Reduced switching in SCID B cells is associated with altered somatic mutation of recombined S regions. J Immunol. 2003;171:6556-64. (FC)
- 12. Martomo SA, Saribasak H, Yokoi M, Hanaoka F, Gearhart PJ. Reevaluation of the role of DNA polymerase θ in somatic hypermutation of immunoglobulin genes. DNA Repair. 2008;7:1603-8. (FC)
- 13. Johansson S, Salmon-Divon M, Johansson MH, Pickman Y, Brodin P, Kärre K, et al. Probing natural killer cell education by Ly49 receptor expression analysis and computational modelling in single MHC class I mice. PLoS One. 2009;4(6):e6046. (FC)
- 14. Brodin P, Lakshmikanth T, Johansson S, Kärre K, Höglund P. The strength of inhibitory input during education quantitatively tunes the functional responsiveness of individual natural killer cells. Blood. 2009;113:2434-41. (FC)
- 15. Pauklin S, Petersen-Mahrt SK. Progesterone inhibits activation-induced deaminase by binding to the promoter. J Immunol. 2009;183:1238-44. (FC)
- 16. Bai L, Deng S, Reboulet R, Mathew R, Teyton L, Savage PB, et al. Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci USA. 2013;110:16097-102. (FC)
- 17. van der Loos C. User Protocol: Practical Guide to Multiple Staining. BioTechniques' Protocol Guide 2010. 2009 Nov. doi: doi10.2144/000113281. (IHC-FS, IHC-PS)
- 18. Halstensen TS, Farstad IN, Scott H, Fausa O, Brandtzaeg P. Intraepithelial TcRα/β+ lymphocytes express CD45RO more often than the TcRγ/δ+ counterparts in coeliac disease. Immunology. 1990;71:460-6. (IHC-FS)
- 19. Ladinser B, Pittschieler K. Expression of the VLA family of integrins in coeliac intestinal mucosa. Acta Paediatr. 1998;87:1125-30. (IHC-FS)
- 20. Richards HB, Satoh M, Jennette JC, Croker BP, Yoshida H, Reeves WH. Interferon-γ is required for lupus nephritis in mice treated with the hydrocarbon oil pristane. Kidney Int. 2001;60:2173-80. (IHC-FS)
- 21. Jacob N, Guo S, Mathian A, Koss MN, Gindea S, Putterman C, et al. B Cell and BAFF dependence of IFN-α-exaggerated disease in systemic lupus erythematosus-prone NZM 2328 mice. J Immunol. 2011;186:4984-93. (IHC-FS)
- 22. Chalan P, Bijzet J, Huitema MG, Kroesen B, Brouwer E, Boots AM. Expression of lectin-like transcript 1, the ligand for CD161, in rheumatoid arthritis. PLoS One. 2015;10(7):e0132436. (IHC-PS)
- 23. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
- 24. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fcγ receptor I. J Immunol. 2015;194:1154-63. (WB)
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