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  • Goat Anti-Mouse IgG2c-BIOT

Goat Anti-Mouse IgG2c-BIOT

Cat. No.:
1078-08
Goat Anti-Mouse IgG2c-Biotin antibody for use in ELISA assays.
$193.00
Size Price (USD) Quantity
1.0 mg $193.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy chain of C57BL/6 mouse IgG2c
Source Pooled antisera from goats hyperimmunized with mouse IgG2c
Cross Adsorption Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2c covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2794463
Gene ID 404711 (Mouse)
Gene ID Symbol Ighg2c (Mouse)
Gene ID Aliases Igh-1b

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2c</sub>-UNLB (SB Cat. No. 0122-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-BIOT (SB Cat. No. 1079-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2c-UNLB (6.3)
    0122-01
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  2. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Ig, Human ads-UNLB (SB Cat. No. 1010-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Mouse Ig, Human ads-UNLB
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
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  4. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human ads-BIOT (SB Cat. No. 1079-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2c, Human ads-BIOT
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  5. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  6. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2a, Human ads-BIOT
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  7. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
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  8. ELISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgM, Human ads-BIOT
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  9. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
    Mouse Anti-Biotin-FITC (SB58c)
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    $165.00
  10. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2b, Human ads-BIOT
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  11. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat IgG-BIOT
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  12. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG3, Human ads-BIOT
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  13. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>2c</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2c</sub>, Human/Bovine/Horse SP ads-BIOT (SB Cat. No. 1077-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG2c, Human/Bovine/Horse SP ads-BIOT
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    $237.00
  14. ELISA plate was coated with purified mouse IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgA-BIOT
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    $193.00
  15. ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG1, Human ads-BIOT
    1070-08
    $226.00
  16. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa-BIOT (SB Cat. No. 1050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse Kappa-BIOT
    1050-08
    $215.00
  17. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-BIOT (SB Cat. No. 1060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse Lambda-BIOT
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  18. ELISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-BIOT (SB Cat. No. 1110-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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