My Cart
$0.00
0

Ensuring timely antibody delivery in the face of supply chain challenges - contact us for solutions

  • Home
  • Goat Anti-Mouse IgG2b, Human ads-APC/CY7

Goat Anti-Mouse IgG2b, Human ads-APC/CY7

Cat. No.:
1090-19
Goat Anti-Mouse IgG2b-APC/CY7 antibody with minimal reactivity to human proteins for use in flow cytometry assays.
$286.00
Size Price (USD) Quantity
0.25 mg $286.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-19
Specificity Reacts with the heavy chain of mouse IgG2b
Source Pooled antisera from goats hyperimmunized with mouse IgG2b
Cross Adsorption Mouse IgG1, IgG2a, IgG3, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG2b covalently linked to agarose
Conjugate APC/CY7 (Allophycocyanin/Cyanine 7)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer
Clonality Polyclonal
Concentration 0.25 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light; Do not freeze
Trademark Information Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH
Applications Quality tested applications for relevant formats include -
ELISA 1-12
FLISA
Flow Cytometry 13-15

Other referenced applications for relevant formats include -
ELISpot 4,6,16
Immunohistochemistry-Frozen Sections 17-19
Immunohistochemistry-Paraffin Sections 17,20
Immunocytochemistry 21,22
Western Blot 23,24
RRID Number AB_2794530
Gene ID 16016 (Mouse)
Gene ID Symbol Ighg2b (Mouse)
Gene ID Aliases IgG2b; Igh-3; gamma2b
UniProt ID P01867 (Mouse
UniProt Name IGG2B_MOUSE (Mouse)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


Need help with this product?
Email technical support
or call 800.722.2255

Other Conjugates and Formulations

Citations

  1. 1. Takahashi I, Marinaro M, Kiyono H, Jackson RJ, Nakagawa I, Fujihashi K, et al. Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin. J Infect Dis. 1996;173:627-35. (ELISA)
  2. 2. Beger E, Deocharan B, Edelman M, Erblich B, Gu Y, Putterman C. A peptide DNA surrogate accelerates autoimmune manifestations and nephritis in lupus-prone mice. J Immunol. 2002;168:3617-26. (ELISA)
  3. 3. Oetke C, Vinson MC, Jones C, Crocker PR. Sialoadhesin-deficient mice exhibit subtle changes in B- and T-cell populations and reduced immunoglobulin M levels. Mol Cell Biol. 2006;26:1549-57. (ELISA)
  4. 4. Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (ELISA, ELISPOT)
  5. 5. Capasso M, Bhamrah MK, Henley T, Boyd RS, Langlais C, Cain K, et al. HVCN1 modulates BCR signal strength via regulation of BCR-dependent generation of reactive oxygen species. Nat Immunol. 2010;11:265-72. (ELISA)
  6. 6. Patterson HC, Kraus M, Wang D, Shahsafaei A, Henderson JM, Seagal J, et al. Cytoplasmic Igα serine/threonines fine-tune Ig α tyrosine phosphorylation and limit bone marrow plasma cell formation. J Immunol. 2011;187:2853-8. (ELISA, ELISPOT)
  7. 7. Futatsugi-Yumikura S, Matsushita K, Fukuoka A, Takahashi S, Yamamoto N, Yonehara S, et al. Pathogenic Th2-type follicular helper T cells contribute to the development of lupus in Fas-deficient mice. Int Immunol. 2014;26:221-31. (ELISA)
  8. 8. Aguilar JL, Varshney AK, Wang X, Stanford L, Scharff M, Fries BC. Detection and measurement of staphylococcal enterotoxin-like K (SEl-K) secretion by Staphylococcus aureus clinical isolates. J Clin Microbiol. 2014;52:2536-43. (ELISA)
  9. 9. Shen P, Roch T, Lampropoulou V, O'Connor RA, Stervbo U, Hilgenberg E, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases. Nature. 2014;507:366-70. (ELISA)
  10. 10. Quakkelaar ED, Fransen MF, van Maren WW, Vaneman J, Loof NM, van Heiningen SH, et al. IgG-mediated anaphylaxis to a synthetic long peptide vaccine containing a B cell epitope can be avoided by slow-release formulation. J Immunol. 2014;192:5813-20. (ELISA)
  11. 11. Patel JM, Vartabedian VF, Kim M, He S, Kang S, Selvaraj P. Influenza virus-like particles engineered by protein transfer with tumor-associated antigens induces protective antitumor immunity. Biotechnol Bioeng. 2015;112:1102-10. (ELISA)
  12. 12. Hartung E, Becker M, Bachem A, Reeg N, Jäkel A, Hutloff A, et al. Induction of potent CD8 T cell cytotoxicity by specific targeting of antigen to cross-presenting dendritic cells in vivo via murine or human XCR1. J Immunol. 2015;194:1069-79. (ELISA)
  13. 13. Gliddon DR, Howard CJ. CD26 is expressed on a restricted subpopulation of dendritic cells in vivo. Eur J Immunol. 2002;32:1472-81. (FC)
  14. 14. Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (FC)
  15. 15. Klausmann S, Sydler T, Summerfield A, Lewis FI, Weilenmann R, Sidler X, et al. T-cell reprogramming through targeted CD4-coreceptor and T-cell receptor expression on maturing thymocytes by latent Circoviridae family member porcine circovirus type 2 cell infections in the thymus. Emerg Microbes Infect. 2015;4:e15. (FC)
  16. 16. Hsu H, Wu Y, Yang P, Wu Q, Job G, Chen J, et al. Overexpression of activation-induced cytidine deaminase in B cells is associated with production of highly pathogenic autoantibodies. J Immunol. 2007;178:5357-65. (ELISPOT)
  17. 17. van der Loos C. User Protocol: Practical Guide to Multiple Staining. BioTechniques' Protocol Guide 2010. 2009 Nov. doi: doi10.2144/000113281. (IHC-FS, IHC-PS)
  18. 18. Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, et al. Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo. J Mol Cell Cardiol. 2006;41:86-96. (IHC-FS)
  19. 19. Jacob N, Guo S, Mathian A, Koss MN, Gindea S, Putterman C, et al. B cell and BAFF dependence of IFN-α-exaggerated disease in systemic lupus erythematosus-prone NZM 2328 mice. J Immunol. 2011;186:4984-93. (IHC-FS)
  20. 20. Huizinga R, van der Star BJ, Kipp M, Jong R, Gerritsen W, Clarner T, et al. Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis. Glia. 2012;60:422-31. (IHC-PS)
  21. 21. Hanon E, Stinchcombe JC, Saito M, Asquith BE, Taylor GP, Tanaka Y, et al. Fratricide among CD8+ T lymphocytes naturally infected with human T cell lymphotropic virus type I. Immunity. 2000;13:657-64. (ICC)
  22. 22. Häggqvist B, Hultman P. Effects of deviating the Th2-response in murine mercury-induced autoimmunity towards a Th1-response. Clin Exp Immunol. 2003;134:202-9. (ICC)
  23. 23. Elkabetz Y, Argon Y, Bar-Nun S. Cysteines in CH1 underlie retention of unassembled Ig heavy chains. J Biol Chem. 2005;280:14402-12. (WB)
  24. 24. Schott C, Graab U, Cuvelier N, Hahn H, Fulda S. Oncogenic RAS mutants confer resistance of RMS13 rhabdomyosarcoma cells to oxidative stress-induced ferroptotic cell death. Front Oncol. 2015;5:131. (WB)
See All References

Related Products

Related Products

Related Products
Check items to add to the cart or
  1. ELISA plate was coated with serially diluted Mouse IgG<sub>2b</sub>-UNLB (SB Cat. No. 0104-01). Immunoglobulin was detected with Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-BIOT (SB Cat. No. 1080-08), Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-BIOT (SB Cat. No. 1090-08), Goat Anti-Mouse IgG<sub>3</sub>, Human ads-BIOT (SB Cat. No. 1100-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Mouse IgG2b-UNLB (A-1)
    0104-01
    $237.00
  2. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2b</sub>-FITC (SB Cat. No. 1091-02).
    Goat Anti-Mouse IgG2b-FITC
    1091-02
    $182.00
  3. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat F(ab')<sub>2</sub> Anti-Mouse IgG<sub>2b</sub>, Human ads-FITC (SB Cat. No. 1092-02) and DAPI.
    Goat F(ab')2 Anti-Mouse IgG2b, Human ads-FITC
    1092-02
    $237.00
  4. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD5-UNLB followed by Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-PE/CY7 (SB Cat. No. 1080-17).
    Goat Anti-Mouse IgG2a, Human ads-PE/CY7
    1080-17
    $248.00
  5. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IgM-UNLB (SB Cat No. 9022-01) followed by Goat Anti-Mouse IgG<sub>3</sub>, Human ads-APC/CY7 (SB Cat. No. 1100-19).
    Goat Anti-Mouse IgG3, Human ads-APC/CY7
    1100-19
    $286.00
  6. Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human/Bovine/Horse SP ads-FITC (SB Cat. No. 1093-02) and DAPI.
    Goat Anti-Mouse IgG2b, Human/Bovine/Horse SP ads-FITC
    1093-02
    $229.00
  7. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD57-UNLB followed by Goat Anti-Mouse IgM, Human ads-PE/CY7 (SB Cat. No. 1020-17).
    Goat Anti-Mouse IgM, Human ads-PE/CY7
    1020-17
    $248.00
  8. FLISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-FITC (SB Cat. No. 1060-02).
    Goat Anti-Mouse Lambda-FITC
    1060-02
    $215.00
  9. ELISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa-BIOT (SB Cat. No. 1050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse Kappa-BIOT
    1050-08
    $215.00
  10. FLISA plate was coated with purified mouse IgE, IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgE-PE (SB Cat. No. 1110-09).
    Goat Anti-Mouse IgE-PE
    1110-09
    $413.00