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Goat Anti-Mouse IgG1, Human ads-BIOT

Goat Anti-Mouse IgG1, Human ads-BIOT

Name: Goat Anti-Mouse IgG1, Human ads-BIOT
SKU: 1070-08
Price: 226 USD
Availability: InStock

Cat. No.:

1070-08

Goat Anti-Mouse IgG₁-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, ELISpot, and multiplex assays.

$226.00

Size	Price (USD)	Quantity
1.0 mg	$226.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) follo

ELISA plate was coated with purified mouse IgG₁, IgG_2a, IgG_2b, IgG₃, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG₁, Human ads-BIOT (SB Cat. No. 1070-08) follo

Human dermal fibroblasts stimulated with unstimulated macrophages for 72 h were stained with anti-collagen type I followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08), CY3 conjugated Streptavidin, and DAPI.<br/>Image from Ploe

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).

Frozen human lung tissue section was stained with anti-DC-SIGN followed by an AP conjugated secondary antibody and New Fucshin. The section was then stained with anti-CD207 followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08)

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	Goat IgG-BIOT
Specificity	Reacts with the heavy chain of mouse IgG₁
Source	Pooled antisera from goats hyperimmunized with mouse IgG₁
Cross Adsorption	Mouse IgG_2a, IgG_2b, IgG₃, IgM, and IgA; human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method	Affinity chromatography on mouse IgG₁ covalently linked to agarose
Conjugate	BIOT (Biotin)
Buffer Formulation	Phosphate buffered saline containing < 0.1% sodium azide
Clonality	Polyclonal
Concentration	0.5 mg/mL
Volume	2.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-8 FLISA Flow Cytometry ^6,9-15 Other referenced applications for relevant formats include - ELISpot ^4,6,7 Immunohistochemistry-Frozen Sections ^3,9,16,17 Immunohistochemistry-Paraffin Sections ^1,16,18,19 Immunohistochemistry-Whole Mount ²⁵ Immunocytochemistry ^14,20,21 Electron Microscopy ¹⁷ Western Blot ^2,5,13,22,23 Surface Plasmon Resonance ²⁴ Multiplex ²⁶
RRID Number	AB_2794413
Gene ID	16017 (Mouse)
Gene ID Symbol	Ighg1 (Mouse)
Gene ID Aliases	IgG1; Igh-4; VH7183
UniProt ID	P01868 (Mouse P01869 (Mouse
UniProt Name	IGHG1_MOUSE (Mouse) IGH1M_MOUSE (Mouse)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

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17. Raats CJ, van den Born J, Bakker MA, Oppers-Walgreen B, Pisa BJ, Dijkman HB, et al. Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies. Am J Pathol. 2000;156:1749-65. (IHC-FS, EM)
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21. Schindler YL, Garske KM, Wang J, Firulli BA, Firulli AB, Poss KD, et al. Hand2 elevates cardiomyocyte production during zebrafish heart development and regeneration. Development. 2014;141:3112-22. (ICC)
22. Konitsiotis AD, Jovanović B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate EW, et al. Topological analysis of Hedgehog acyltransferase, a multipalmitoylated transmembrane protein. J Biol Chem. 2015;290:3293-307. (WB)
23. Esser-von Bieren J, Volpe B, Kulagin M, Sutherland DB, Guiet R, Seitz A, et al. Antibody-mediated trapping of helminth larvae requires CD11b and Fcγ receptor I. J Immunol. 2015;194:1154-63. (WB)
24. Sun Y, Peng I, Senger K, Hamidzadeh K, Reichelt M, Baca M, et al. Critical role of activation induced cytidine deaminase in experimental autoimmune encephalomyelitis. Autoimmunity. 2013;46:157-67. (Surface Plasmon Resonance)
25. Zhu D, Fang Y, Gao K, Shen J, Zhong TP, Li F. Vegfa impacts early myocardium development in zebrafish. Int J Mol Sci. 2017;18:444. (IHC-WM)
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