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  • Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-UNLB

Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-UNLB

Cat. No.:
1037-01
Purified Goat Anti-Mouse IgG(H+L) antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA and immunocytochemistry assays.
$77.00
Size Price (USD) Quantity
1.0 mg $77.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
RRID Number AB_2794354

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. SouthernBiotech published data (ICC)

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  2. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  5. BALB/c mouse splenocytes were stained with Goat Anti-Mouse Kappa-UNLB (SB Cat. No. 1050-01) follow by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-FITC (SB Cat. No. 6300-02).
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  6. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human, rat, hamster, goat, sheep, rabbit, chicken, guinea pig, horse, and bovine serum. Immunoglobulins and sera were detected with serially diluted Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-UNLB (SB Cat. No. 1038-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  7. Lane 1 - Mouse IgG<sub>1</sub>κ<br/>Lane 2 - Mouse IgG<sub>2a</sub>λ<br/>Lane 3 - Mouse IgG<sub>2b</sub>κ<br/>Lane 4 - Mouse IgG<br/>Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Mouse Kappa-HRP (SB Cat. No. 1050-05; 1:4K). Proteins were visualized using chemiluminescent detection.
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  8. Paraffin embedded mouse spleen section was stained with Goat Anti-Mouse Kappa-UNLB (SB Cat. No. 1050-01) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP (SB Cat. No. 6420-04), Red AP, and hematoxylin.
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  9. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Rat ads-UNLB (SB Cat. No. 1034-01) then Mouse Anti-Goat IgG Fc-FITC.
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  10. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  11. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L), Human ads-UNLB (SB Cat. No. 1031-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  12. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  13. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF647 (SB Cat. No. 6425-31).
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  14. Lane 1 - Mouse IgG<sub>1</sub>κ<br/>Lane 2 - Mouse IgG<sub>2a</sub>λ<br/>Lane 3 - Mouse IgG<sub>2b</sub>κ<br/>Mouse immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Goat Anti-Mouse Lambda-HRP (SB Cat. No. 1060-05; 1:4K). Proteins were visualized using chemiluminescent detection.
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  15. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L)-UNLB (SB Cat. No. 1036-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  16. ELISA plate was coated with purified mouse IgG, IgM, and IgA and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  17. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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