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  • Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-BIOT

Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-BIOT

Cat. No.:
1037-08
Goat Anti-Mouse IgG(H+L)-Biotin antibody with minimal reactivity to human, bovine, and horse serum proteins for use in ELISA and immunocytochemistry assays.
$105.00
Size Price (USD) Quantity
1.0 mg $105.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
RRID Number AB_2794358

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. SouthernBiotech published data (ICC)

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  1. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  2. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 1038-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT
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  3. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6415-08), Streptavidin-TXRD (SB Cat. No. 7100-07), and DAPI.
    Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT
    6415-08
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  5. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L)-BIOT (SB Cat. No. 1036-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Mouse IgG(H+L)-BIOT
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  7. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
    Mouse Anti-Biotin-FITC (SB58c)
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  8. Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Goat Anti-Rat IgG(H+L), Mouse ads-BIOT
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  10. Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.<br/>Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca<sup>2+</sup>/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT
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  11. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat Anti-Mouse IgG(H+L), Human ads-BIOT
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  12. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Rat ads-BIOT (SB Cat. No. 1034-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat Anti-Mouse IgG(H+L), Rat ads-BIOT
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  13. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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