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  • Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-AF555

Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-AF555

Cat. No.:
1037-32
Goat Anti-Mouse IgG(H+L)-Alexa Fluor® 555 antibody with minimal reactivity to human, bovine, and horse serum proteins for use in immunocytochemistry and other fluorescent assays.
$156.00
Size Price (USD) Quantity
1.0 mg $156.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
RRID Number AB_2794359

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. SouthernBiotech published data (ICC)

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  1. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 1038-32) and DAPI.
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  2. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  3. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) and Rabbit IgG-UNLB (SB Cat. No. 0111-01; left) followed by Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-AF488 (SB Cat. No. 6442-30) and DAPI.
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  4. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  5. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L)-AF555 (SB Cat. No. 1036-32).
    Goat Anti-Mouse IgG(H+L)-AF555
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  6. HCC827 cells were stained with anti-E-cadherin followed by a secondary antibody and mounted with DAPI Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-20).<br/>Image from Narita M, Shimura E, Nagasawa A, Aiuchi T, Suda Y, Hamada Y, et al. Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c. PLoS One. 2017;12(2):e0172115. Figure 4(e)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. Frozen mouse skeletal muscle tissue was stained with anti-myosin heavy chain and anti-myosin heavy chain type I followed by Goat Anti-Mouse IgG, Human ads-AF555 (SB Cat. No. 1030-32) and Goat Anti-Mouse IgM-FITC (SB Cat. No. 1021-02).<br/>Image from Cortez-Toledo O, Schnair C, Sangngern P, Metzger D, Chao LC. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS One. 2017;12(2):e0171268. Figure 3(a)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-AF555 (SB Cat. No. 6415-32) and DAPI.
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  9. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Rat ads-AF555 (SB Cat. No. 1034-32) and DAPI.
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  10. FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 6425-30).
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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