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  • Goat Anti-Mouse IgG(H+L), Human ads-AF647

Goat Anti-Mouse IgG(H+L), Human ads-AF647

Cat. No.:
1031-31
Goat Anti-Mouse IgG(H+L)-Alexa Fluor® 647 antibody with minimal reactivity to human proteins for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$132.00
Size Price (USD) Quantity
1.0 mg $132.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF647
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA
Source Pooled antisera from goats hyperimmunized with mouse IgG
Cross Adsorption Human immunoglobulins and pooled sera; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-7
FLISA 8
Flow Cytometry 3,8-13

Other referenced applications for relevant formats include -
ELISpot 28
Immunohistochemistry-Frozen Sections 14,15
Immunohistochemistry-Paraffin Sections 16
Immunocytochemistry 12,17-20
Western Blot 21-27
RRID Number AB_2794316

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Swiecki MK, Lisanby MW, Shu F, Turnbough CL Jr, Kearney JF. Monoclonal antibodies for Bacillus anthracis spore detection and functional analyses of spore germination and outgrowth. J Immunol. 2006;176:6076-84. (ELISA)
  2. 2. Stegmann T, Kamphuis T, Meijerhof T, Goud E, de Haan A, Wilschut J. Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation. Vaccine. 2010;28:5543-50. (ELISA)
  3. 3. Christianson GJ, Sun VZ, Akilesh S, Pesavento E, Proetzel G, Roopenian DC. Monoclonal antibodies directed against human FcRn and their applications. MAbs. 2012;4:208-16. (ELISA, FC)
  4. 4. Stefka AT, Feehley T, Tripathi P, Qiu J, McCoy K, Mazmanian SK, et al. Commensal bacteria protect against food allergen sensitization. Proc Natl Acad Sci USA. 2014;111:13145-50. (ELISA)
  5. 5. Silva-Sanchez A, Liu CR, Vale AM, Khass M, Kapoor P, Elgavish A, et al. Violation of an evolutionarily conserved immunoglobulin diversity gene sequence preference promotes production of dsDNA-specific IgG antibodies. PLoS One. 2015;10(2):e0118171. (ELISA)
  6. 6. Lascano V, Hahne M, Papon L, Cameron K, Röeder C, Schafmayer C, et al. Circulating APRIL levels are correlated with advanced disease and prognosis in rectal cancer patients. Oncogenesis. 2015;4:e136. (ELISA)
  7. 7. Zhao H, Bauzon F, Bi E, Yu JJ, Fu H, Lu Z, et al. Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age. J Biol Chem. 2015;290:5797-809. (ELISA)
  8. 8. Kilpatrick KE, Cutler T, Whitehorn E, Drape RJ, Macklin MD, Witherspoon SM, et al. Gene gun delivered DNA-based immunizations mediate rapid production of murine monoclonal antibodies to the Flt-3 receptor. Hybridoma. 1998;17:569-76. (FLISA, FC)
  9. 9. Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW. Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J Immunol. 2005;175:7325-31. (FC)
  10. 10. Donini M, Zenaro E, Tamassia N, Dusi S. NADPH oxidase of human dendritic cells: role in Candida albicans killing and regulation by interferons, dectin-1 and CD206. Eur J Immunol. 2007;37:1194-203. (FC)
  11. 11. Delaleu N, Madureira AC, Immervoll H, Jonsson R. Inhibition of experimental Sjögren's syndrome through immunization with HSP60 and its peptide amino acids 437-460. Arthritis Rheum. 2008;58:2318-28. (FC)
  12. 12. Le TL, Mironova E, Garcin D, Compans RW. Induction of influenza-specific mucosal immunity by an attenuated recombinant Sendai virus. PLoS One. 2011;6:e18780. (FC, ICC)
  13. 13. Daflon-Yunes N, Pinto-Silva FE, Vidal RS, Novis BF, Berguetti T, Lopes RR, et al. Characterization of a multidrug-resistant chronic myeloid leukemia cell line presenting multiple resistance mechanisms. Mol Cell Biochem. 2013;383:123-35. (FC)
  14. 14. Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (IHC-FS)
  15. 15. Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (IHC-FS)
  16. 16. Deckert V, Kretz B, Habbout A, Raghay K, Labbé J, Abello N, et al. Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency. Am J Pathol. 2013;183:975-86. (IHC-PS)
  17. 17. Scaife S, Brown R, Kellie S, Filer A, Martin S, Thomas AM, et al. Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display. Rheumatology. 2004;43:1346-52. (ICC)
  18. 18. Thomas RA, Norman JC, Huynh TT, Williams B, Bolton SJ, Wardlaw AJ. Mechanical stretch has contrasting effects on mediator release from bronchial epithelial cells, with a rho-kinase-dependent component to the mechanotransduction pathway. Respir Med. 2006;100:1588-97. (ICC)
  19. 19. Lee S, Bahn JH, Choi CK, Whitlock NC, English AE, Safe S, et al. ESE-1/EGR-1 pathway plays a role in tolfenamic acid-induced apoptosis in colorectal cancer cells. Mol Cancer Ther. 2008;7:3739-50. (ICC)
  20. 20. Tarafdar S, Poe JA, Smithgall TE. The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem. 2014;289:15718-28. (ICC)
  21. 21. Dalen KT, Ulven SM, Arntsen BM, Solaas K, Nebb HI. PPARα activators and fasting induce the expression of adipose differentiation-related protein in liver. J Lipid Res. 2006;47:931-43. (WB)
  22. 22. Hangen E, De Zio D, Bordi M, Zhu C, Dessen P, Caffin F, et al. A brain-specific isoform of mitochondrial apoptosis-inducing factor: AIF2. Cell Death Differ. 2010;17:1155-66. (WB)
  23. 23. Lunde IG, Kvaløy H, Austbø B, Christensen G, Carlson CR. Angiotensin II and norepinephrine activate specific calcineurin-dependent NFAT transcription factor isoforms in cardiomyocytes. J Appl Physiol. 2011;111:1278-89. (WB)
  24. 24. Norheim F, Raastad T, Thiede B, Rustan AC, Drevon CA, Haugen F. Proteomic identification of secreted proteins from human skeletal muscle cells and expression in response to strength training. Am J Physiol Endocrinol Metab. 2011;301:E1013-21. (WB)
  25. 25. Thomas AP, Dunn TN, Drayton JB, Oort PJ, Adams SH. A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone. Nutr Metab. 2012;9:3. (WB)
  26. 26. Stuckenholz C, Lu L, Thakur PC, Choi T, Shin D, Bahary N. Sfrp5 modulates both Wnt and BMP signaling and regulates gastrointestinal organogenesis in the zebrafish, Danio rerio. PLoS One. 2013;8(4):e62470. (WB)
  27. 27. Muller M, Hutin S, Marigold O, Li KH, Burlingame A, Glaunsinger BA. A ribonucleoprotein complex protects the interleukin-6 mRNA from degradation by distinct herpesviral endonucleases. PLoS Pathog. 2015;11(5):e1004899. (WB)
  28. 28. Rahman ZS, Niu H, Perry D, Wakeland E, Manser T, Morel L. Expression of the autoimmune Fcgr2b NZW allele fails to be upregulated in germinal center B cells and is associated with increased IgG production. Genes Immun. 2007;8:604-12. (ELISPOT)
See All References

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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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