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  • Goat Anti-Mouse IgA-TXRD

Goat Anti-Mouse IgA-TXRD

Cat. No.:
1040-07
Goat Anti-Mouse IgA-Texas Red antibody for use in flow cytometry and immunohistochemistry / immunocytochemistry assays.
$193.00
Size Price (USD) Quantity
1.0 mg $193.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-TXRD
Specificity Reacts with the heavy chain of mouse IgA
Source Pooled antisera from goats hyperimmunized with mouse IgA
Cross Adsorption Mouse IgG and IgM; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgA covalently linked to agarose
Conjugate TXRD (Texas Red®)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Texas Red® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-15,23
FLISA
Flow Cytometry 1,5,6,10,13,17-20,23,24

Other referenced applications for relevant formats include -
ELISpot 2,7,14,16,23
Immunohistochemistry-Frozen Sections 3,4,9,11
Immunohistochemistry-Paraffin Sections 5,6
Immunocytochemistry 21,24
Western Blot 8,12,22
Separation 1
Depletion 23
RRID Number AB_2794373
Gene ID 238447 (Mouse)
Gene ID Symbol Igha (Mouse)
Gene ID Aliases IgA; Igh-2

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-9. (ELISA, FC, Sep)
  2. 2. van Ginkel FW, Wahl SM, Kearney JF, Kweon M, Fujihashi K, Burrows PD, et al. Partial IgA-deficiency with increased Th2-type cytokines in TGF-β1 knockout mice. J Immunol. 1999;163:1951-7. (ELISA, ELISPOT)
  3. 3. Pappo J, Torrey D, Castriotta L, Savinainen A, Kabok Z, Ibraghimov A. Helicobacter pylori infection in immunized mice lacking major histocompatibility complex class I and class II functions. Infect Immun. 1999;67:337-41. (ELISA, IHC-FS)
  4. 4. Dunn LA, Upcroft JA, Fowler EV, Matthews BS, Upcroft P. Orally administered Giardia duodenalis extracts enhance an antigen-specific antibody response. Infect Immun. 2001;69:6503-10. (ELISA, IHC-FS)
  5. 5. Ibnou-Zekri N, Blum S, Schiffrin EJ, von der Weid T. Divergent patterns of colonization and immune response elicited from two intestinal Lactobacillus strains that display similar properties in vitro. Infect Immun. 2003;71:428-36. (ELISA, FC, IHC-PS)
  6. 6. Wang J, Anders RA, Wu Q, Peng D, Cho JH, Sun Y, et al. Dysregulated LIGHT expression on T cells mediates intestinal inflammation and contributes to IgA nephropathy. J Clin Invest. 2004;113:826-35. (ELISA, FC, IHC-PS)
  7. 7. Hovden A, Brokstad KA, Major D, Wood J, Haaheim LR, Cox RJ. A pilot study of the immune response to whole inactivated avian influenza H7N1 virus vaccine in mice. Influenza Other Respir Viruses. 2009;3:21-8. (ELISA, ELISPOT)
  8. 8. Sanchez CJ, Kumar N, Lizcano A, Shivshankar P, Hotopp JC, Jorgensen JH, et al. Streptococcus pneumoniae in biofilms are unable to cause invasive disease due to altered virulence determinant production. PLoS One. 2011;6(12):e28738. (ELISA, WB)
  9. 9. Harris DP, Vogel P, Wims M, Moberg K, Humphries J, Jhaver KG, et al. Requirement for class II phosphoinositide 3-kinase C2α in maintenance of glomerular structure and function. Mol Cell Biol. 2011;31:63-80. (ELISA, IHC-FS)
  10. 10. Willems KN. Regulation of humoral immunity by Pim kinases [dissertation]. Worcester (USA): University of Massachusetts; 2011. (ELISA, FC)
  11. 11. Zhang H, Jia C, Xi H, Li S, Yang L, Wang Y. Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice. Exp Eye Res. 2011;93:50-8. (ELISA, IHC-FS)
  12. 12. Nakanishi K, Narimatsu S, Ichikawa S, Tobisawa Y, Kurohane K, Niwa Y, et al. Production of hybrid-IgG/IgA plantibodies with neutralizing activity against Shiga toxin 1. PLoS One. 2013;8(11):e80712. (ELISA, WB)
  13. 13. Goverse G, Olivier BJ, Molenaar R, Knippenberg M, Greuter M, Konijn T, et al. Vitamin A metabolism and mucosal immune function are distinct between BALB/c and C57BL/6 mice. Eur J Immunol. 2015;45:89-100. (ELISA, FC)
  14. 14. Surman SL, Jones BG, Rudraraju R, Sealy RE, Hurwitz JL. Intranasal administration of retinyl palmitate with a respiratory virus vaccine corrects impaired mucosal IgA response in the vitamin A-deficient host. Clin Vaccine Immunol. 2014; 21:598-601. (ELISA, ELISPOT)
  15. 15. Pucella JN, Yen W, Kim MV, van der Veeken J, Socci ND, Naito Y, et al. miR-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. J Immunol. 2015;194:2635-42. (ELISA)
  16. 16. Patel PS, Kearney JF. Neonatal exposure to pneumococcal phosphorylcholine modulates the development of house dust mite allergy during adult life. J Immunol. 2015;194:5838-50. (ELISPOT)
  17. 17. Jiang H, Bos NA, Cebra JJ. Timing, localization, and persistence of colonization by segmented filamentous bacteria in the neonatal mouse gut depend on immune status of mothers and pups. Infect Immun. 2001;69:3611-7. (FC)
  18. 18. Cook AJ, Oganesian L, Harumal P, Basten A, Brink R, Jolly CJ. Reduced switching in SCID B cells is associated with altered somatic mutation of recombined S regions. J Immunol. 2003;171:6556-64. (FC)
  19. 19. Guikema JE, Schrader CE, Leus NG, Ucher A, Linehan EK, Werling U, et al. Reassessment of the role of Mut S homolog 5 in Ig class switch recombination shows lack of involvement in cis- and trans-switching. J Immunol. 2008;181:8450-9. (FC)
  20. 20. Pauklin S, Petersen-Mahrt SK. Progesterone inhibits activation-induced deaminase by binding to the promoter. J Immunol. 2009;183:1238-44. (FC)
  21. 21. Armitage CW, O'Meara CP, Harvie MC, Timms P, Wijburg OL, Beagley KW. Evaluation of intra- and extra-epithelial secretory IgA in chlamydial infections. Immunology. 2014;143:520-30. (ICC)
  22. 22. Endt K, Stecher B, Chaffron S, Slack E, Tchitchek N, Benecke A, et al. The microbiota mediates pathogen clearance from the gut lumen after non-typhoidal Salmonella diarrhea. PLoS Pathog. 2010;6(9):e1001097. (WB)
  23. 23. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. J Immunol. 1988;140:3033-9. (ELISA, FC, ELISPOT, Depletion)
  24. 24. Usui T, Wakatsuki Y, Matsunaga Y, Kaneko S, Koseki H, Kita T, et al. Overexpression of B cell-specific activator protein (BSAP/Pax-5) in a late B cell is sufficient to suppress differentiation to an Ig high producer cell with plasma cell phenotype. J Immunol. 1997;158:3197-204. (FC, ICC)
See All References

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  2. FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07).
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  3. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  4. Frozen mouse spleen tissue was stained with Goat Anti-Mouse IgM, Human ads-TXRD (SB Cat. No. 1020-07) and Rat Anti-Mouse IgD-BIOT (SB Cat. No. 1120-08) followed by Streptavidin-AF488.<br/>Image from Ren W, Lagerstedt K, Grimsholm O, Stern A, Sun J, Fang Y, et al. Uncoupling of natural IgE production and CD23 surface expression levels. PLoS One. 2013;8(4):e62851. Figure 2(c)<br/>Reproduced under the Creative Commons Attribution License
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  5. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  6. FLISA plate was coated with purified mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgM-TXRD (SB Cat. No. 1021-07).
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  7. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>1</sub>, Human ads-TXRD (SB Cat. No. 1070-07).
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  8. FLISA plate was coated with purified mouse IgG<sub>1</sub>, IgG<sub>2a</sub>, IgG<sub>2b</sub>, IgG<sub>3</sub>, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG<sub>2a</sub>, Human ads-TXRD (SB Cat. No. 1080-07).
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  9. Human fibroblast-like synoviocytes were stained with anti-RelA followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-FITC (SB Cat. No. 4050-02) and DAPI.<br/>Image from Crowley T, O'Neil JD, Adams H, Thomas AM, Filer A, Buckley CD, et al. Priming in response to pro-inflammatory cytokines is a feature of adult synovial but not dermal fibroblasts. Arthritis Res Ther. 2017;19:35. Figure S1<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  10. Frozen section of TEPC 1165 cell line pellet was stained with Goat Anti-Mouse Kappa-TXRD (SB Cat. No. 1050-07).<br/>Image from Wang H, Owens JD, Shih JH, Li M, Bonner RF, Mushinski JF. Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA. BMC Genomics. 2006;7:97. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  11. FLISA plate was coated with purified mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Lambda-TXRD (SB Cat. No. 1060-07).
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