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  • Goat Anti-Human Lambda, Mouse ads-AF647

Goat Anti-Human Lambda, Mouse ads-AF647

Cat. No.:
2071-31
Goat Anti-Human Lambda-Alexa Fluor® 647 antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.
$429.00
Size Price (USD) Quantity
1.0 mg $429.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF647
Specificity Reacts with human λ light chains
Source Pooled antisera from goats hyperimmunized with human λ light chains
Cross Adsorption Human κ light chains; mouse immunoglobulins and pooled sera; may react with λ light chains from other species
Purification Method Affinity chromatography on human λ light chains covalently linked to agarose
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry

RRID Number AB_2895754
Gene ID 3535 (Human)
Gene ID Symbol IGL (Human)
Gene ID Aliases IGL@; IGLC6

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-AF647 (SB Cat. No. 2070-31).
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  2. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa, Mouse ads-AF647 (SB Cat. 2061-31) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).
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  3. FLISA plate was coated with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-UNLB (SB Cat. No. 6300-01). Serially diluted Goat IgG-AF647 (SB Cat. No. 0109-31) was captured and fluorescence intensity quantified.
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  4. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-AF647 (SB Cat. No. 2060-31).
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  6. Goat F(ab')2 Anti-Human Lambda-AF647
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  7. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-AF647 (SB Cat. No. 2062-31).
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  8. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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