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  • Goat Anti-Human Lambda-FITC

Goat Anti-Human Lambda-FITC

Cat. No.:
2070-02
Goat Anti-Human Lambda-FITC antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, and western blot assays.
$149.00
Size Price (USD) Quantity
1.0 mg $149.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with human λ light chains
Source Pooled antisera from goats hyperimmunized with human λ light chains
Cross Adsorption Human κ light chains; may react with λ light chains from other species
Purification Method Affinity chromatography on human λ light chains covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA 1-11
FLISA
Flow Cytometry 18-20

Other referenced applications for relevant formats include -
ELISpot 12
Immunohistochemistry-Paraffin Sections 13
Immunocytochemistry 14-16
Western Blot 16
Immunoprecipitation 17
Surface Plasmon Resonance 21
Stimulation 22
RRID Number AB_2795750
Gene ID 3535 (Human)
Gene ID Symbol IGL (Human)
Gene ID Aliases IGL@; IGLC6

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Sy MS, Guo Y, Stamenkovic I. Distinct effects of two CD44 isoforms on tumor growth in vivo. J Exp Med. 1991;174:859-66. (ELISA)
  2. 2. Houpt DC, Pfrommer GS, Young BJ, Larson TA, Kozel TR. Occurrences, immunoglobulin classes, and biological activities of antibodies in normal human serum that are reactive with Cryptococcus neoformans glucuronoxylomannan. Infect Immun. 1994;62:2857-64. (ELISA)
  3. 3. Deng Y, Notkins AL. Molecular determinants of polyreactive antibody binding: HCDR3 and cyclic peptides. Clin Exp Immunol. 2000;119:69-76. (ELISA)
  4. 4. Pancook JD, Beuerlein G, Pecht G, Tang Y, Nie Y, Wu H, et al. In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens. Hybrid Hybridomics. 2001;20:383-96. (ELISA)
  5. 5. Hinton PR, Johlfs MG, Xiong JM, Hanestad K, Ong KC, Bullock C, et al. Engineered human IgG antibodies with longer serum half-lives in primates. J Biol Chem. 2004;279:6213-6. (ELISA)
  6. 6. Tsurushita N, Park M, Pakabunto K, Ong K, Avdalovic A, Fu H, et al. Humanization of a chicken anti-IL-12 monoclonal antibody. J Immunol Methods. 2004;295:9-19. (ELISA)
  7. 7. Hinton PR, Xiong JM, Johlfs MG, Tang MT, Keller S, Tsurushita N. An engineered human IgG1 antibody with longer serum half-life. J Immunol. 2006;176:346-56. (ELISA)
  8. 8. Sydor JR, Normant E, Pien CS, Porter JR, Ge J, Grenier L, et al. Development of 17-allylamino-17-demethoxygeldanamycin hydroquinone hydrochloride (IPI-504), an anti-cancer agent directed against Hsp90. Proc Natl Acad Sci USA. 2006;103:17408-13. (ELISA)
  9. 9. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (ELISA)
  10. 10. Guan Y, Sajadi MM, Kamin-Lewis R, Fouts TR, Dimitrov A, Zhang Z, et al. Discordant memory B cell and circulating anti-Env antibody responses in HIV-1 infection. Proc Natl Acad Sci USA. 2009;106:3952-7. (ELISA)
  11. 11. Xu Z, Juan V, Ivanov A, Ma Z, Polakoff D, Powers DB, et al. Affinity and cross-reactivity engineering of CTLA4-Ig to modulate T cell costimulation. J Immunol. 2012;189:4470-7. (ELISA)
  12. 12. Saletti G, Çuburu N, Yang JS, Dey A, Czerkinsky C. Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood. Nat Protoc. 2013;8:1073-87. (ELISPOT)
  13. 13. Bonda DJ, Webber KM, Siedlak SL, Perry G, Friedland RP, Smith MA. The pathology of Alzheimer disease elicits an in vivo immunological response. Am J Immunol. 2007;3:10-14. (IHC-PS)
  14. 14. Campana D, Coustan-Smith E, Janossy G. The immunologic detection of minimal residual disease in acute leukemia. Blood. 1990;76:163-71. (ICC)
  15. 15. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
  16. 16. Hasegawa H, Forte C, Barber I, Turnbaugh S, Stoops J, Shen M, et al. Modulation of in vivo IgG crystallization in the secretory pathway by heavy chain isotype class switching and N-linked glycosylation. Biochim Biophys Acta. 2014;1843:1325-38. (ICC, WB)
  17. 17. Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, et al. FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol. 2011;23:43-53. (IP)
  18. 18. Kumagai M, Coustan-Smith E, Murray DJ, Silvennoinen O, Murti KG, Evans WE, et al. Ligation of CD38 suppresses human B lymphopoiesis. J Exp Med. 1995;181:1101-10. (FC)
  19. 19. Minegishi Y, Conley ME. Negative selection at the pre-BCR checkpoint elicited by human μ heavy chains with unusual CDR3 regions. Immunity. 2001;14:631-41. (FC)
  20. 20. Dühren-von Minden M, Übelhart R, Schneider D, Wossning T, Bach MP, Buchner M, et al. Chronic lymphocytic leukaemia is driven by antigen-independent cell-autonomous signalling. Nature. 2012;489:309-12. (FC)
  21. 21. Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, et al. Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity. Proc Natl Acad Sci USA. 2014;111:16820-5. (Surface Plasmon Resonance)
  22. 22. Nechansky A, Robertson MW, Albrecht BA, Apgar JR, Kricek F. Inhibition of antigen-induced mediator release from IgE-sensitized cells by a monoclonal anti-FcεRI α-chain receptor antibody: implications for the involvement of the membrane-proximal α-chain region in FcεRI-mediated cell activation. J Immunol. 2001;166:5979-90. (Stim)
See All References

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  1. Frozen mouse lymph node section was stained with Goat IgG-FITC isotype control (SB Cat. No. 0109-02; left) and Goat Anti-Mouse IgM, Human ads-FITC (SB Cat. No. 1020-02; right) followed by DAPI.
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  2. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa-FITC (SB Cat. 2060-02) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2071-02).
    Goat Anti-Human Lambda, Mouse ads-FITC
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  5. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda-FITC (SB Cat. No. 2072-02).
    Goat F(ab')2 Anti-Human Lambda-FITC
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  6. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Mouse Anti-Human Lambda-FITC (SB Cat. No. 9180-02).
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  7. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-FITC (SB Cat. No. 2073-02).
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  8. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa, Mouse ads-FITC (SB Cat. No. 2061-02).
    Goat Anti-Human Kappa, Mouse ads-FITC
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  9. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-FITC (SB Cat. No. 2062-02).
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  10. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa, Mouse ads-FITC (SB Cat. No. 2063-02).
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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