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  • Goat Anti-Human Kappa-UNLB

Goat Anti-Human Kappa-UNLB

Cat. No.:
2060-01
Purified Goat Anti-Human Kappa antibody for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, western blot, immunoprecipitation, ELISpot, purification, and stimulation assays and for immobilization in surface plasmon resonance.
$121.00
Size Price (USD) Quantity
1.0 mg $121.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-UNLB
Specificity Reacts with human κ light chains
Source Pooled antisera from goats hyperimmunized with human κ light chains
Cross Adsorption Human λ light chains; may react with κ light chains from other species
Purification Method Affinity chromatography on human κ light chains covalently linked to agarose
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1-9
FLISA
Flow Cytometry 19-22

Other referenced applications for relevant formats include -
ELISpot 10
Immunohistochemistry-Paraffin Sections 11
Immunocytochemistry 12
Western Blot 4,13-17
Immunoprecipitation 18
Surface Plasmon Resonance 23
Purification 24
Stimulation 25
RRID Number AB_2795716
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Pancook JD, Beuerlein G, Pecht G, Tang Y, Nie Y, Wu H, et al. In vitro affinity maturation of human IgM antibodies reactive with tumor-associated antigens. Hybrid Hybridomics. 2001;20:383-96. (ELISA)
  2. 2. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (ELISA)
  3. 3. Guan Y, Sajadi MM, Kamin-Lewis R, Fouts TR, Dimitrov A, Zhang Z, et al. Discordant memory B cell and circulating anti-Env antibody responses in HIV-1 infection. Proc Natl Acad Sci USA. 2009;106:3952-7. (ELISA)
  4. 4. Lai H, Engle M, Fuchs A, Keller T, Johnson S, Gorlatov S, et al. Monoclonal antibody produced in plants efficiently treats West Nile virus infection in mice. Proc Natl Acad Sci USA. 2010;107:2419-24. (ELISA, WB)
  5. 5. Boross P, Lohse S, Nederend M, Jansen JH, van Tetering G, Dechant M, et al. IgA EGFR antibodies mediate tumour killing in vivo. EMBO Mol Med. 2013;5:1213-26. (ELISA)
  6. 6. Kehoe JW, Whitaker B, Bethea D, Lacy ER, Boakye K, Santulli-Marotto S, et al. Isolation and optimization for affinity and biophysical characteristics of anti-CCL17 antibodies from the VH1-69 germline gene. Protein Eng Des Sel. 2014;27:199-206. (ELISA)
  7. 7. Wyrzucki A, Dreyfus C, Kohler I, Steck M, Wilson IA, Hangartner L. Alternative recognition of the conserved stem epitope in influenza A virus hemagglutinin by a VH3-30-encoded heterosubtypic antibody. J Virol. 2014;88:7083-92. (ELISA)
  8. 8. Nagano R, Masuda K. Establishment of a signal peptide with cross-species compatibility for functional antibody expression in both Escherichia coli and Chinese hamster ovary cells. Biochem Biophys Res Commun. 2014;447:655-9. (ELISA)
  9. 9. London AS, Mackay K, Lihon M, He Y. Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations. Biotechnol Prog. 2014;30:1497-1501. (ELISA)
  10. 10. Saletti G, Çuburu N, Yang JS, Dey A, Czerkinsky C. Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood. Nat Protoc. 2013;8:1073-87. (ELISPOT)
  11. 11. Bonda DJ, Webber KM, Siedlak SL, Perry G, Friedland RP, Smith MA. The pathology of Alzheimer disease elicits an in vivo immunological response. Am J Immunol. 2007;3:10-14. (IHC-PS)
  12. 12. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
  13. 13. Singh G, Parker S, Hobart P. The development of a bicistronic plasmid DNA vaccine for B-cell lymphoma. Vaccine. 2002;20:1400-11. (WB)
  14. 14. Corisdeo S, Wang B. Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditions. Protein Expr Purif. 2004;34:270-9. (WB)
  15. 15. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (WB)
  16. 16. He J, Peng L, Lai H, Hurtado J, Stahnke J, Chen Q. A plant-produced antigen elicits potent immune responses against West Nile virus in mice. Biomed Res Int. 2014;2014:952865. (WB)
  17. 17. Helman D, Toister-Achituv M, Bar-Shimon M, Cohen B, Otmi I, Berger N, et al. Novel membrane-bound reporter molecule for sorting high producer cells by flow cytometry. Cytometry A. 2014;85:162-8. (WB)
  18. 18. Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, et al. FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol. 2011;23:43-53. (IP)
  19. 19. Minegishi Y, Conley ME. Negative selection at the pre-BCR checkpoint elicited by human μ heavy chains with unusual CDR3 regions. Immunity. 2001;14:631-41. (FC)
  20. 20. Hinton PR, Xiong JM, Johlfs MG, Tang MT, Keller S, Tsurushita N. An engineered human IgG1 antibody with longer serum half-life. J Immunol. 2006;176:346-56. (FC)
  21. 21. Dong J, Sereno A, Aivazian D, Langley E, Miller BR, Snyder WB, et al. A stable IgG-like bispecific antibody targeting the epidermal growth factor receptor and the type I insulin-like growth factor receptor demonstrates superior anti-tumor activity. mAbs. 2011;3:273-88. (FC)
  22. 22. Xiang Y, Park S, Garrard WT. A major deletion in the V-J intervening region results in hyperelevated transcription of proximal V genes and a severely restricted repertoire. J Immunol. 2014;193:3746-54. (FC)
  23. 23. Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, et al. Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity. Proc Natl Acad Sci USA. 2014;111:16820-5. (Surface Plasmon Resonance)
  24. 24. Damschroder MM, Widjaja L, Gill PS, Krasnoperov V, Jiang W, Dall'Acqua WF, et al. Framework shuffling of antibodies to reduce immunogenicity and manipulate functional and biophysical properties. Mol Immunol. 2007;44:3049-60. (Purification)
  25. 25. Su K, Li X, Edberg JC, Wu J, Ferguson P, Kimberly RP. A promoter haplotype of the immunoreceptor tyrosine-based inhibitory motif-bearing FcγRIIb alters receptor expression and associates with autoimmunity. II. Differential binding of GATA4 and Yin-Yang1 transcription factors and correlated receptor expression and function. J Immunol. 2004;172:7192-9. (Stim)
See All References

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  1. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  2. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-UNLB (SB Cat. No. 2070-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  5. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  8. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human Kappa-UNLB (SB Cat. No. 2062-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  17. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  18. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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