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Goat Anti-Human IgM-HRP

Goat Anti-Human IgM-HRP

Name: Goat Anti-Human IgM-HRP
SKU: 2020-05
Price: 99 USD
Availability: InStock

Cat. No.:

2020-05

Goat Anti-Human IgM-HRP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays.

$99.00

Size	Price (USD)	Quantity
1.0 mL	$99.00

Qty

Technical Bulletin Safety Datasheet

ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-HRP (SB Cat. No. 2020-05).

Product Specifications

More Information
Isotype	Goat IgG
Isotype Control	0109-05
Specificity	Reacts with the heavy chain of human IgM
Source	Pooled antisera from goats hyperimmunized with human IgM
Cross Adsorption	Human IgG and IgA; may react with IgM from other species
Purification Method	Affinity chromatography on human IgM covalently linked to agarose
Conjugate	HRP (Horseradish Peroxidase)
Buffer Formulation	50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality	Polyclonal
Concentration	Lot specific
Volume	1.0 mL
Recommended Storage	2-8°C
Applications	Quality tested applications for relevant formats include - ELISA ^1-3,15 FLISA Flow Cytometry ^16-18 Other referenced applications for relevant formats include - ELISpot ^2,4,5,11 Immunohistochemistry-Frozen Sections ^6,7 Immunohistochemistry-Paraffin Sections ^8,9 Immunocytochemistry ^8,10,11 Western Blot ^12,13 Immunoprecipitation ^13-15 Multiplex ^19,20 Stimulation ^21-23 Purification ²⁴ Depletion ²⁵
RRID Number	AB_2795603
Gene ID	3507 (Human)
Gene ID Symbol	IGHM (Human)
Gene ID Aliases	AGM1; MU; VH
UniProt ID	P01871 (Human
UniProt Name	IGHM_HUMAN (Human)

Documentation

Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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Other Conjugates and Formulations

Citations

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7. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-6. (IHC-FS)
8. Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992;175:397-404. (IHC-PS, ICC)
9. Rowley AH, Shulman ST, Mask CA, Finn LS, Terai M, Baker SC, et al. IgA plasma cell infiltration of proximal respiratory tract, pancreas, kidney, and coronary artery in acute Kawasaki disease. J Infect Dis. 2000;182:1183-91. (IHC-PS)
10. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
11. Alwayn IP, Xu Y, Basker M, Wu C, Buhler L, Lambrigts D, et al. Effects of specific anti-B and/or anti-plasma cell immunotherapy on antibody production in baboons: depletion of CD20- and CD22-positive B cells does not result in significantly decreased production of anti-αGal antibody. Xenotransplantation. 2001;8:157-71. (ELISPOT, ICC)
12. Djoumerska IK, Tchorbanov AI, Donkova-Petrini VD, Pashov AD, Vassilev TL. Serum IgM, IgG and IgA block by F(ab')₂-dependent mechanism the binding of natural IgG autoantibodies from therapeutic immunoglobulin preparations to self-antigens. Eur J Haematol. 2005;74:101-10. (WB)
13. Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, et al. FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol. 2011;23:43-53. (IP, WB)
14. Pollok BA, Anker R, Eldridge P, Hendershot L, Levitt D. Molecular basis of the cell-surface expression of immunoglobulin μ chain without light chain in human B lymphocytes. Proc Natl Acad Sci USA. 1987;84:9199-203. (IP)
15. Minegishi Y, Conley ME. Negative selection at the pre-BCR checkpoint elicited by human μ heavy chains with unusual CDR3 regions. Immunity. 2001;14:631-41. (ELISA, IP)
16. Mongini PK, Blessinger C, Posnett DN, Rudich SM. Membrane IgD and membrane IgM differ in capacity to transduce inhibitory signals within the same human B cell clonal populations. J Immunol. 1989;143:1565-74. (FC)
17. Dobbs AK, Yang T, Farmer D, Kager L, Parolini O, Conley ME. Cutting edge: a hypomorphic mutation in Igβ (CD79b) in a patient with immunodeficiency and a leaky defect in B cell development. J Immunol. 2007;179:2055-9. (FC)
18. Azimzadeh AM, Byrne GW, Ezzelarab M, Welty E, Braileanu G, Cheng X, et al. Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells. Xenotransplantation. 2014;21:555-66. (FC)
19. Tomaras GD, Yates NL, Liu P, Qin L, Fouda GG, Chavez LL, et al. Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. J Virol. 2008;82:12449-63. (Multiplex)
20. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)
21. Blaeser F, Ho N, Prywes R, Chatila TA. Ca²⁺-dependent gene expression mediated by MEF2 transcription factors. J Biol Chem. 2000;275:197-209. (Stim)
22. Batlle A, Papadopoulou V, Gomes AR, Willimott S, Melo JV, Naresh K, et al. CD40 and B-cell receptor signalling induce MAPK family members that can either induce or repress Bcl-6 expression. Mol Immunol. 2009;46:1727-35. (Stim)
23. Woyach JA, Bojnik E, Ruppert AS, Stefanovski MR, Goettl VM, Smucker KA, et al. Bruton's tyrosine kinase (BTK) function is important to the development and expansion of chronic lymphocytic leukemia (CLL). Blood. 2014;123:1207-13. (Stim)
24. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (Purification)
25. Palaia JM, McConnell M, Achenbach JE, Gustafson CE, Stoermer KA, Nolan M, et al. Neutralization of HIV subtypes A and D by breast milk IgG from women with HIV infection in Uganda. J Infect. 2014;68:264-72. (Depletion)