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  • Goat Anti-Human IgM-AF555

Goat Anti-Human IgM-AF555

Cat. No.:
2020-32
Goat Anti-Human IgM-Alexa Fluor® 555 antibody for use in immunohistochemistry / immunocytochemistry and western blot assays.
$139.00
Size Price (USD) Quantity
1.0 mg $139.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control 0109-32
Specificity Reacts with the heavy chain of human IgM
Source Pooled antisera from goats hyperimmunized with human IgM
Cross Adsorption Human IgG and IgA; may react with IgM from other species
Purification Method Affinity chromatography on human IgM covalently linked to agarose
Conjugate AF555 (Alexa Fluor® 555)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1-3,15
FLISA
Flow Cytometry 16-18

Other referenced applications for relevant formats include -
ELISpot 2,4,5,11
Immunohistochemistry-Frozen Sections 6,7
Immunohistochemistry-Paraffin Sections 8,9
Immunocytochemistry 8,10,11
Western Blot 12,13
Immunoprecipitation 13-15
Multiplex 19,20
Stimulation 21-23
Purification 24
Depletion 25
RRID Number AB_2795609
Gene ID 3507 (Human)
Gene ID Symbol IGHM (Human)
Gene ID Aliases AGM1; MU; VH
UniProt ID P01871 (Human
UniProt Name IGHM_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Houpt DC, Pfrommer GS, Young BJ, Larson TA, Kozel TR. Occurrences, immunoglobulin classes, and biological activities of antibodies in normal human serum that are reactive with Cryptococcus neoformans glucuronoxylomannan. Infect Immun. 1994;62:2857-64. (ELISA)
  2. 2. Traggiai E, Volpi S, Schena F, Gattorno M, Ferlito F, Moretta L, et al. Bone marrow-derived mesenchymal stem cells induce both polyclonal expansion and differentiation of B cells isolated from healthy donors and systemic lupus erythematosus patients. Stem Cells. 2008;26:562-9. (ELISA, ELISPOT)
  3. 3. Yang JS, Kang S, Yun C, Han SH. Evaluation of anticoagulants for serologic assays of cholera vaccination. Clin Vaccine Immunol. 2014;21:854-8. (ELISA)
  4. 4. Friman V, Quiding M, Czerkinsky C, Nordström I, Larsson L, Ericson D, et al. Intestinal and circulating antibody-forming cells in IgA-deficient individuals after oral cholera vaccination. Clin Exp Immunol. 1994;95:222-6. (ELISPOT)
  5. 5. Lynch RJ, Silva IA, Chen BJ, Punch JD, Cascalho M, Platt JL. Cryptic B cell response to renal transplantation. Am J Transplant. 2013;13:1713-23. (ELISPOT)
  6. 6. Vajdy M, Veazey RS, Knight HK, Lackner AA, Neutra MR. Differential effects of simian immunodeficiency virus infection on immune inductive and effector sites in the rectal mucosa of rhesus macaques. Am J Pathol. 2000;157:485-95. (IHC-FS)
  7. 7. Forshammar J, Isaksson S, Strid H, Stotzer P, Sjövall H, Simrén M, et al. A pilot study of colonic B cell pattern in irritable bowel syndrome. Scand J Gastroenterol. 2008;43:1461-6. (IHC-FS)
  8. 8. Solvason N, Kearney JF. The human fetal omentum: a site of B cell generation. J Exp Med. 1992;175:397-404. (IHC-PS, ICC)
  9. 9. Rowley AH, Shulman ST, Mask CA, Finn LS, Terai M, Baker SC, et al. IgA plasma cell infiltration of proximal respiratory tract, pancreas, kidney, and coronary artery in acute Kawasaki disease. J Infect Dis. 2000;182:1183-91. (IHC-PS)
  10. 10. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
  11. 11. Alwayn IP, Xu Y, Basker M, Wu C, Buhler L, Lambrigts D, et al. Effects of specific anti-B and/or anti-plasma cell immunotherapy on antibody production in baboons: depletion of CD20- and CD22-positive B cells does not result in significantly decreased production of anti-αGal antibody. Xenotransplantation. 2001;8:157-71. (ELISPOT, ICC)
  12. 12. Djoumerska IK, Tchorbanov AI, Donkova-Petrini VD, Pashov AD, Vassilev TL. Serum IgM, IgG and IgA block by F(ab')2-dependent mechanism the binding of natural IgG autoantibodies from therapeutic immunoglobulin preparations to self-antigens. Eur J Haematol. 2005;74:101-10. (WB)
  13. 13. Santiago T, Kulemzin SV, Reshetnikova ES, Chikaev NA, Volkova OY, Mechetina LV, et al. FCRLA is a resident endoplasmic reticulum protein that associates with intracellular Igs, IgM, IgG and IgA. Int Immunol. 2011;23:43-53. (IP, WB)
  14. 14. Pollok BA, Anker R, Eldridge P, Hendershot L, Levitt D. Molecular basis of the cell-surface expression of immunoglobulin μ chain without light chain in human B lymphocytes. Proc Natl Acad Sci USA. 1987;84:9199-203. (IP)
  15. 15. Minegishi Y, Conley ME. Negative selection at the pre-BCR checkpoint elicited by human μ heavy chains with unusual CDR3 regions. Immunity. 2001;14:631-41. (ELISA, IP)
  16. 16. Mongini PK, Blessinger C, Posnett DN, Rudich SM. Membrane IgD and membrane IgM differ in capacity to transduce inhibitory signals within the same human B cell clonal populations. J Immunol. 1989;143:1565-74. (FC)
  17. 17. Dobbs AK, Yang T, Farmer D, Kager L, Parolini O, Conley ME. Cutting edge: a hypomorphic mutation in Igβ (CD79b) in a patient with immunodeficiency and a leaky defect in B cell development. J Immunol. 2007;179:2055-9. (FC)
  18. 18. Azimzadeh AM, Byrne GW, Ezzelarab M, Welty E, Braileanu G, Cheng X, et al. Development of a consensus protocol to quantify primate anti-non-Gal xenoreactive antibodies using pig aortic endothelial cells. Xenotransplantation. 2014;21:555-66. (FC)
  19. 19. Tomaras GD, Yates NL, Liu P, Qin L, Fouda GG, Chavez LL, et al. Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. J Virol. 2008;82:12449-63. (Multiplex)
  20. 20. Pochechueva T, Chinarev A, Bovin N, Fedier A, Jacob F, Heinzelmann-Schwarz V. PEGylation of microbead surfaces reduces unspecific antibody binding in glycan-based suspension array. J Immunol Methods. 2014;412:42-52. (Multiplex)
  21. 21. Blaeser F, Ho N, Prywes R, Chatila TA. Ca2+-dependent gene expression mediated by MEF2 transcription factors. J Biol Chem. 2000;275:197-209. (Stim)
  22. 22. Batlle A, Papadopoulou V, Gomes AR, Willimott S, Melo JV, Naresh K, et al. CD40 and B-cell receptor signalling induce MAPK family members that can either induce or repress Bcl-6 expression. Mol Immunol. 2009;46:1727-35. (Stim)
  23. 23. Woyach JA, Bojnik E, Ruppert AS, Stefanovski MR, Goettl VM, Smucker KA, et al. Bruton's tyrosine kinase (BTK) function is important to the development and expansion of chronic lymphocytic leukemia (CLL). Blood. 2014;123:1207-13. (Stim)
  24. 24. Janoff EN, Fasching C, Orenstein JM, Rubins JB, Opstad NL, Dalmasso AP. Killing of Streptococcus pneumoniae by capsular polysaccharide-specific polymeric IgA, complement, and phagocytes. J Clin Invest. 1999;104:1139-47. (Purification)
  25. 25. Palaia JM, McConnell M, Achenbach JE, Gustafson CE, Stoermer KA, Nolan M, et al. Neutralization of HIV subtypes A and D by breast milk IgG from women with HIV infection in Uganda. J Infect. 2014;68:264-72. (Depletion)
See All References

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