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  • Goat Anti-Human IgG, Mouse/Bovine/Horse SP ads-BIOT

Goat Anti-Human IgG, Mouse/Bovine/Horse SP ads-BIOT

Cat. No.:
2045-08
Goat Anti-Human IgG-Biotin antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in flow cytometry and ELISA assays.
$193.00
Size Price (USD) Quantity
1.0 mg $193.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the heavy chain of human IgG
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Human IgM and IgA; mouse, bovine, and horse serum proteins (SP); may react with IgG from other species
Purification Method Affinity chromatography on human IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
Flow Cytometry

RRID Number AB_2895743

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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  1. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  2. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat IgG-BIOT
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  3. ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (SB Cat. No. 2049-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgG, Monkey ads-BIOT
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  4. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Human IgG-BIOT (SB Cat. No. 2042-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat F(ab')2 Anti-Human IgG-BIOT
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  5. ELISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-BIOT (SB Cat. No. 2050-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgA-BIOT
    2050-08
    $99.00
  6. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  7. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgG Fc-BIOT (JDC-10)
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  8. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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  9. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  10. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
    Streptavidin-AP
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  11. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-BIOT (SB Cat. No. 2020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgM-BIOT
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  12. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
    Mouse Anti-Biotin-PE (SB58c)
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  13. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Kappa-BIOT (SB Cat. No. 2060-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Kappa-BIOT
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  14. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9042-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Human IgG Fc-BIOT (H2)
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  15. ELISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-BIOT (SB Cat. No. 2070-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human Lambda-BIOT
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  16. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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  17. ELISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgD-BIOT (SB Cat. No. 2030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Human IgD-BIOT
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  18. ELISA plate was coated with serially diluted Human IgG Fc-UNLB (SB Cat. No. 0160-01). Immunoglobulin was detected with Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08), Goat Anti-Human Ig Fab-BIOT (SB Cat. No. 2085-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT and Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9130-08 and Cat. No. 9140-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Human IgG Fc-UNLB
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