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  • Goat Anti-Human IgG, Mouse ads-AF647

Goat Anti-Human IgG, Mouse ads-AF647

Cat. No.:
2044-31
Goat Anti-Human IgG-Alexa Fluor® 647 antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.
$259.00
Size Price (USD) Quantity
1.0 mg $259.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF647
Specificity Reacts with the heavy chain of human IgG
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Human IgM and IgA; mouse immunoglobulins and pooled sera; may react with IgG from other species
Purification Method Affinity chromatography on human IgG covalently linked to agarose
Conjugate AF647 (Alexa Fluor® 647)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA 1
FLISA
Flow Cytometry

RRID Number AB_2895740

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Caven TH, Sturgill JL, Conrad DH. BCR ligation antagonizes the IL-21 enhancement of anti-CD40/IL-4 plasma cell differentiation and IgE production found in low density human B cell cultures. Cell Immunol. 2007;247:49-58. (ELISA)

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  2. FLISA plate was coated with Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-UNLB (SB Cat. No. 6300-01). Serially diluted Goat IgG-AF647 (SB Cat. No. 0109-31) was captured and fluorescence intensity quantified.
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  7. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
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