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  • Goat Anti-Human IgG Fc, Mouse/Bovine/Horse SP ads-BIOT

Goat Anti-Human IgG Fc, Mouse/Bovine/Horse SP ads-BIOT

Cat. No.:
2081-08
Goat Anti-Human IgG Fc-Biotin antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in ELISA assays.
$94.00
Size Price (USD) Quantity
0.5 mg $94.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-BIOT
Specificity Reacts with the Fc region of human IgG
Source Pooled antisera from goats hyperimmunized with human IgG
Cross Adsorption Human IgG Fab, IgM, and IgA; mouse, bovine, and horse serum proteins (SP); may react with IgG from other species
Purification Method Affinity chromatography on human IgG Fc covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895757

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG Fc-BIOT (SB Cat. No. 2048-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  2. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat IgG-BIOT (SB Cat No. 0109-08; gray) and Goat Anti-Mouse IgG<sub>1</sub>, Human ads-BIOT (SB Cat. No. 1070-08) then Streptavidin-FITC (SB Cat. No. 7100-02).
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  3. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  4. ELISA plate was coated with purified human IgG, IgM, and IgA, cynomolgus IgG, and rhesus IgG. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Monkey ads-BIOT (SB Cat. No. 2049-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  5. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  6. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG Fc, Multi-Species SP ads-BIOT (SB Cat. No. 2014-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  7. Human IgG-UNLB (SB Cat. No. 0150-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Mouse Anti-Human IgG Fc-UNLB (SB Cat. No. 9040-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05) secondary antibody and chemiluminescent detection.
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  8. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG Fc, Mouse/Bovine/Horse SP ads-HRP (SB Cat. No. 2081-05).
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  9. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  10. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse/Bovine/Horse SP ads-HRP (SB Cat. No. 2045-05).
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  11. ELISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM-BIOT (SB Cat. No. 2020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  12. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  13. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse ads-HRP (SB Cat. No. 2044-05).
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  14. ELISA plate was coated with serially diluted Human IgG<sub>1</sub> Kappa-UNLB (SB Cat. No. 0151K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  15. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  16. ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG Fc-BIOT (SB Cat. No. 9042-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  21. ELISA plate was coated with purified human IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Human Ig Fc-BIOT (SB Cat. No. 2047-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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