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  • Goat Anti-Human IgD-AF488

Goat Anti-Human IgD-AF488

Cat. No.:
2030-30
Goat Anti-Human IgD-Alexa Fluor® 488 antibody for use in flow cytometry and immunohistochemistry assays.
$185.00
Size Price (USD) Quantity
1.0 mg $185.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-AF488
Specificity Reacts with the heavy chain of human IgD
Source Pooled antisera from goats hyperimmunized with human IgD
Cross Adsorption Human IgG, IgM, and IgA; may react with IgD from other species
Purification Method Affinity chromatography on human IgD covalently linked to agarose
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA
Flow Cytometry 5-17

Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 1-4
Immunohistochemistry-Paraffin Sections 19
Separation 18
RRID Number AB_2795631
Gene ID 3495 (Human)
Gene ID Symbol IGHD (Human)
UniProt ID P01880 (Human
UniProt Name IGHD_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Pospisil R, Silverman GJ, Marti GE, Aruffo A, Bowen MA, Mage RG. CD5 is a potential selecting ligand for B-cell surface immunoglobulin: a possible role in maintenance and selective expansion of normal and malignant B cells. Leuk Lymphoma. 2000;36:353-65. (IHC-FS)
  2. 2. Hirbod T, Kaldensjö T, Broliden K. In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa. PLoS One. 2011;6(9):e25551. (IHC-FS)
  3. 3. Rakhmanov M, Sic H, Kienzler A, Fischer B, Rizzi M, Seidl M, et al. High levels of SOX5 decrease proliferative capacity of human B cells, but permit plasmablast differentiation. PLoS One. 2014;9(6):e100328. (IHC-FS)
  4. 4. Cols M, Barra CM, He B, Puga I, Xu W, Chiu A, et al. Stromal endothelial cells establish a bidirectional crosstalk with chronic lymphocytic leukemia cells through the TNF-related factors BAFF, APRIL, and CD40L. J Immunol. 2012;188:6071-83. (IHC-FS)
  5. 5. Mongini PK, Blessinger C, Posnett DN, Rudich SM. Membrane IgD and membrane IgM differ in capacity to transduce inhibitory signals within the same human B cell clonal populations. J Immunol. 1989;143:1565-74. (FC)
  6. 6. Kumagai M, Coustan-Smith E, Murray DJ, Silvennoinen O, Murti KG, Evans WE, et al. Ligation of CD38 suppresses human B lymphopoiesis. J Exp Med. 1995;181:1101-10. (FC)
  7. 7. Klein U, Rajewsky K, Küppers R. Human immunoglobulin (Ig)M+IgD+ peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells. J Exp Med. 1998;188:1679-89. (FC)
  8. 8. van der Loo JC, Hanenberg H, Cooper RJ, Luo F, Lazaridis EN, Williams DA. Nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse as a model system to study the engraftment and mobilization of human peripheral blood stem cells. Blood. 1998;92:2556-70. (FC)
  9. 9. Bräuninger A, Goossens T, Rajewsky K, Küppers R. Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human. Eur J Immunol. 2001;31:3631-7. (FC)
  10. 10. Fournillier A, Freida D, Defrance T, Merle P, Trépo C, Inchauspé G. Analysis of B-lymphocyte differentiation in patients infected with hepatitis C virus. J Med Virol. 2004;72:566-74. (FC)
  11. 11. Johansen F, Baekkevold ES, Carlsen HS, Farstad IN, Soler D, Brandtzaeg P. Regional induction of adhesion molecules and chemokine receptors explains disparate homing of human B cells to systemic and mucosal effector sites: dispersion from tonsils. Blood. 2005;106:593-600. (FC)
  12. 12. Dryer RL, Covey LR. Use of chromatin immunoprecipitation (ChIP) to detect transcription factor binding to highly homologous promoters in chromatin isolated from unstimulated and activated primary human B cells. Biol Proced Online. 2006;8:44-54. (FC)
  13. 13. Sira MM, Yoshida T, Takeuchi M, Kashiwayama Y, Futatani T, Kanegane H, et al. A novel immunoregulatory protein in human colostrum, syntenin-1, for promoting the development of IgA-producing cells from cord blood B cells. Int Immunol. 2009;21:1013-23. (FC)
  14. 14. Petrovas C, Yamamoto T, Gerner MY, Boswell KL, Wloka K, Smith EC, et al. CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest. 2012;122:3281-94. (FC)
  15. 15. Pallikkuth S, Micci L, Ende ZS, Iriele RI, Cervasi B, Lawson B, et al. Maintenance of intestinal Th17 cells and reduced microbial translocation in SIV-infected rhesus macaques treated with interleukin (IL)-21. PLoS One. 2013;9(7):e1003471. (FC)
  16. 16. Yammani RD, Haas KM. Primate B-1 cells generate antigen-specific B cell responses to T cell-independent type 2 antigens. J Immunol. 2013;190:3100-8. (FC)
  17. 17. Neumann B, Klippert A, Raue K, Sopper S, Stahl-Hennig C. Characterization of B and plasma cells in blood, bone marrow, and secondary lymphoid organs of rhesus macaques by multicolor flow cytometry. J Leukoc Biol. 2015;97:19-30. (FC)
  18. 18. Joseph AM, Babcock GJ, Thorley-Lawson DA. Cells expressing the Epstein-Barr virus growth program are present in and restricted to the naive B-cell subset of healthy tonsils. J Virol. 2000;74:9964-71. (Sep)
  19. 19. SouthernBiotech unpublished data
See All References

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