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Goat Anti-Human IgA, Mouse/Bovine/Horse SP ads-FITC

Cat. No.:
2053-02
Goat Anti-Human IgA-FITC antibody with minimal reactivity to mouse, bovine, and horse serum proteins for use in fluorescent assays.
$209.00
Size Price (USD) Quantity
1.0 mg $209.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Goat IgG
Isotype Control Goat IgG-FITC
Specificity Reacts with the heavy chain of human IgA
Source Pooled antisera from goats hyperimmunized with human IgA
Cross Adsorption Human IgG and IgM; mouse, bovine, and horse serum proteins (SP); may react with IgA from other species
Purification Method Affinity chromatography on human IgA covalently linked to agarose
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_3076612

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. FLISA plate was coated with purified human IgA, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Human IgA-FITC (SB Cat. No. 2050-02).
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  2. FLISA plate was coated with purified human IgA<sub>1</sub>, IgA<sub>2</sub>, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA<sub>1</sub>-FITC (SB Cat. No. 9130-02).
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  3. ELISA plate was coated with serially diluted Human IgA Kappa-UNLB (SB Cat. No. 0155K-01). Immunoglobulin was detected with Mouse Anti-Human IgG<sub>1</sub> Hinge-BIOT (SB Cat. No. 9052-08), Mouse Anti-Human IgG<sub>2</sub> Fc-BIOT (SB Cat. No. 9060-08), Mouse Anti-Human IgG<sub>3</sub> Hinge-BIOT (SB Cat. No. 9210-08), Mouse Anti-Human IgG<sub>4</sub> pFc'-BIOT (SB Cat. No. 9190-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  4. FLISA plate was coated with purified human IgA<sub>1</sub>, IgA<sub>2</sub>, IgD, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgA<sub>2</sub>-FITC (SB Cat. No. 9140-02).
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  5. ELISA plate was coated with serially diluted Human IgA Lambda-UNLB (SB Cat. No. 0155L-01). Immunoglobulin was detected with Mouse Anti-Human IgG-BIOT (SB Cat. No. 9040-08), Mouse Anti-Human IgM-BIOT (SB Cat. No. 9020-08), Mouse Anti-Human IgA<sub>1</sub>-BIOT (SB Cat. No. 9130-08), Mouse Anti-Human IgA<sub>2</sub>-BIOT (SB Cat. No. 9140-08), Mouse Anti-Human IgD-BIOT (SB Cat. No. 9030-08), Mouse Anti-Human IgE Fc-BIOT (SB Cat. No. 9160-08), Mouse Anti-Human Kappa-BIOT (SB Cat. No. 9230-08), and Mouse Anti-Human Lambda-BIOT (SB Cat. No. 9180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.
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  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-FITC (SB Cat. No. 2040-02).
    Goat Anti-Human IgG-FITC
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  8. CD19+ human B-lymphocytes were stained with Goat Anti-Human Kappa-FITC (SB Cat. 2060-02) and Goat F(ab')<sub>2</sub> Anti-Human Lambda, Mouse ads-PE (SB Cat. No. 2073-09).
    Goat Anti-Human Kappa-FITC
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  9. Human pancreatic carcinoma cell line MIA PaCa-2 was blocked with 10% Normal Goat Serum (SB Cat. No. 0060-01) and stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF555 (SB Cat. No. 1031-32) and DAPI.
    Normal Goat Serum
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  10. FLISA plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-FITC (SB Cat. No. 2070-02).
    Goat Anti-Human Lambda-FITC
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  11. FLISA plate was coated with purified human IgD, IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgD-FITC (SB Cat. No. 2030-02).
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  12. FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-FITC (SB Cat. No. 2023-02).
    Goat Anti-Human IgM, Mouse/Bovine/Horse SP ads-FITC
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  13. FLISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG, Mouse/Bovine/Horse SP ads-FITC (SB Cat. No. 2045-02).
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