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  • Donkey F(ab')2 Anti-Rat IgG(H+L), Mouse SP ads-HRP

Donkey F(ab')2 Anti-Rat IgG(H+L), Mouse SP ads-HRP

Cat. No.:
6431-05
Donkey F(ab')2 Anti-Rat IgG(H+L)-HRP antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.
$171.00
Size Price (USD) Quantity
1.0 mL $171.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pepsin digest of Donkey Anti-Rat IgG(H+L), Mouse SP ads (SB Cat. No. 6430)
Cross Adsorption Mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796361

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rat Ig, Mouse ads-HRP (SB Cat. No. 3010-05) and chemiluminescent detection.<br/>Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
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  3. Donkey Anti-Rat IgG(H+L), Mouse SP ads-HRP
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  4. Spleen, brain, and lung tissues from wild type (+/+) and homozygous Limk2 (-/-) mutant mice were resolved by electrophoresis, transfered to PVDF membrane, and probed with anti-LIMK2 followed by Goat Anti-Rat IgG(H+L), Mouse ads-HRP (SB Cat. No. 3050-05) and chemiluminescent detection.<br/>Image from Rice DS, Hansen GM, Liu F, Crist MJ, Newhouse MM, Potter D, et al. Keratinocyte migration in the developing eyelid requires LIMK2. PLoS One. 2012;7(10):e47168. Figure 1(c)<br/>Reproduced under the Creative Commons Attribution License
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  5. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L), Human ads-HRP (SB Cat. No. 6185-05).
    Rabbit Anti-Rat IgG(H+L), Human ads-HRP
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  6. ELISA plate was coated with purified rat IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgM-HRP (SB Cat. No. 3020-05).
    Goat Anti-Rat IgM-HRP
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  7. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  8. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-HRP (SB Cat. No. 6180-05).
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  9. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 3051-05).
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  10. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Rat IgG(H+L)-HRP (SB Cat. No. 6130-05).
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  11. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-HRP (SB Cat. No. 3030-05).
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    Rat IgM-UNLB (NIP/M-2)
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