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  • Donkey F(ab')2 Anti-Rat IgG(H+L), Mouse SP ads-BIOT

Donkey F(ab')2 Anti-Rat IgG(H+L), Mouse SP ads-BIOT

Cat. No.:
6431-08
Donkey F(ab')2 Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to mouse serum proteins for use in ELISA assays.
$151.00
Size Price (USD) Quantity
0.5 mg $151.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM
Source Pepsin digest of Donkey Anti-Rat IgG(H+L), Mouse SP ads (SB Cat. No. 6430)
Cross Adsorption Mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rat immunoglobulins
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895820

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT
    6430-08
    $122.00
  2. Frozen BALB/c mouse intestine tissue was stained with Rat IgG<sub>2a</sub>-UNLB isotype control (SB Cat. No. 0117-01; left) and Rat Anti-Mouse CD104-UNLB (SB Cat. No. 1855-01; right) followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3050-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Goat Anti-Rat IgG(H+L), Mouse ads-BIOT
    3050-08
    $192.00
  3. Normal Donkey Serum
    Normal Donkey Serum
    0030-01
    $47.00
  4. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
    7105-05
    $128.00
  5. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Rat Anti-Mouse IgA-UNLB (SB Cat. No. 1165-01) followed by Rabbit Anti-Rat IgG(H+L), Human ads-BIOT (SB Cat. No. 6185-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
    Rabbit Anti-Rat IgG(H+L), Human ads-BIOT
    6185-08
    $122.00
  6. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 3051-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rat IgG(H+L), Mouse/Human ads-BIOT
    3051-08
    $151.00
  7. ELISA plate was coated with serially diluted Rat IgG-UNLB (SB Cat. No. 0108-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Mouse Anti-Rat IgM-BIOT (SB Cat. No. 3080-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rat IgG-UNLB
    0108-01
    $122.00
  8. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with Rabbit Anti-Rat IgG(H+L)-BIOT (SB Cat. No. 6180-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Rabbit Anti-Rat IgG(H+L)-BIOT
    6180-08
    $81.00
  9. Frozen rat gastrocnemic muscle section was stained with Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by a CY3 secondary reagent and DAPI.<br/>Image from Duehrkop C, Banz Y, Spirig R, Miescher S, Nolte MW, Spycher M, et al. C1 esterase inhibitor reduces lower extremity ischemia/reperfusion injury and associated lung damage. PLoS One. 2013;8(8):e72059. Figure 2(h)<br/>Reproduced under the Creative Commons Attribution License
    Goat Anti-Rat IgM-BIOT
    3020-08
    $128.00
  10. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rat IgG-BIOT
    3030-08
    $139.00
  11. ELISA plate was coated with serially diluted Rat IgM-UNLB (SB Cat. No. 0120-01). Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT (SB Cat. No. 3030-08) and Goat Anti-Rat IgM-BIOT (SB Cat. No. 3020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rat IgM-UNLB (NIP/M-2)
    0120-01
    $137.00
  12. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
    Mouse Anti-Biotin-FITC (SB58c)
    6404-02
    $174.00
  13. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45-UNLB (SB Cat. No. 1660-01) followed by Goat F(ab')<sub>2</sub> Anti-Rat IgG(H+L), Mouse ads-BIOT (SB Cat. No. 3052-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
    Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads-BIOT
    3052-08
    $157.00
  14. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
    Streptavidin-AP
    7105-04
    $128.00
  15. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins were detected with serially diluted Goat Fab Anti-Rat Ig, Mouse ads-BIOT (SB Cat. No. 3000-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Fab Anti-Rat Ig, Mouse ads-BIOT
    3000-08
    $177.00
  16. ELISA plate was coated with purified rat IgG and IgM. Immunoglobulins and serum were detected with serially diluted Mouse Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 3053-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Rat IgG(H+L), Mouse SP ads-BIOT
    3053-08
    $134.00