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  • Donkey F(ab')2 Anti-Rabbit IgG(H+L), Multi-Species SP ads-BIOT

Donkey F(ab')2 Anti-Rabbit IgG(H+L), Multi-Species SP ads-BIOT

Cat. No.:
6444-08
Donkey F(ab')2 Anti-Rabbit IgG(H+L)-Biotin antibody with minimal reactivity to human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins for use in ELISA assays.
$149.00
Size Price (USD) Quantity
0.5 mg $149.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pepsin digest of Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads (SB Cat. No. 6442)
Cross Adsorption Human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796389

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4052-08) followed Streptavidin-HRP (SB Cat. No. 7100-05).
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  2. Normal Donkey Serum
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  3. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  4. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-BIOT (SB Cat. No. 4054-08) followd by Streptavidin-HRP (SB Cat. No. 7100-05).
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  5. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgM-BIOT
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  6. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
    Rabbit IgG-UNLB
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  7. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-BIOT (SB Cat. No. 4010-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit Ig, Human ads-BIOT
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  8. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  9. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L)-BIOT (SB Cat. No. 6441-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  10. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-BIOT (SB Cat. No. 4091-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-AP (SB Cat. No. 7105-04).
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  12. ELISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG Fc-BIOT
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  13. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG-BIOT
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  14. Rabbit peripheral blood lymphocytes were stained with Rat Anti-Rabbit Ig-BIOT (SB Cat. No. 4065-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
    Rat Anti-Rabbit Ig-BIOT (SB87a)
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  15. FLISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>2b</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Mouse Anti-Biotin-FITC (SB Cat. No. 6404-02).
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  16. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rabbit IgM-UNLB
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  17. Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control (SB Cat. No. 0111-01; left) and Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01; right) followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT (SB Cat. No. 6440-08, Streptavidin-CY3 (SB Cat. No. 7100-12), and DAPI.
    Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT
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  18. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-BIOT (SB Cat. No. 4090-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Rabbit IgG-BIOT (2A9)
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  19. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab-BIOT (SB Cat. No. 4040-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Goat Anti-Rabbit IgG Fab-BIOT
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  20. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  21. Chicken sperm was stained with anti-CaMKIV followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-BIOT (SB Cat. No. 4050-08), Streptavidin-CY2 (SB Cat. No. 7100-21), and DAPI.<br/>Image from Nguyen TM, Combarnous Y, Praud C, Duittoz A, Blesbois E. Ca<sup>2+</sup>/calmodulin-dependent protein kinase kinases (CaMKKs) effects on AMP-activated protein kinase (AMPK) regulation of chicken sperm functions. PLoS One. 2016;11(1):e0147559. Figure 4(f)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  22. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6442-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads-BIOT
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