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  • Donkey F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-UNLB

Donkey F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-UNLB

Cat. No.:
6446-01
Purified Donkey F(ab')2 Anti-Rabbit IgG(H+L) antibody with minimal reactivity to human, mouse, and rat serum proteins for use in ELISA assays.
$108.00
Size Price (USD) Quantity
0.5 mg $108.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pepsin digest of Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads (SB Cat. No. 6440)
Cross Adsorption Mouse, rat, and human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796391

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with purified rabbit IgG and IgM, mouse IgG, IgM, and IgA, and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-UNLB (SB Cat. No. 4052-01) followed Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
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  2. Normal Donkey Serum
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  3. ELISA plate was coated with purified rabbit IgG and IgM and human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum. Immunoglobulins and sera were detected with serially diluted Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB (SB Cat. No. 6444-01) followed by Goat Anti-Equine IgG(H+L)-HRP (SB Cat. No. 6040-05).
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  4. Frozen mouse lung tissue section following 21 days of hypoxia was stained with anti-S100A4 followed by Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-UNLB (SB Cat. No. 4050-01) followed by an AP conjugated secondary antibody and a substrate for the detection of alkaline phosphatase.<br/>Image from Kwapiszewska G, Wilhelm J, Wolff S, Laumanns I, Koenig IR, Ziegler A, et al. Expression profiling of laser-microdissected intrapulmonary arteries in hypoxia-induced pulmonary hypertension. Respir Res. 2005;6:109. Figure 5(b)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
    Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-UNLB
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  5. FLISA plate was coated with purified feline IgG. Immunoglobulin was detected with Goat Anti-Feline IgG(H+L)-FITC (SB Cat. No. 6080-02).
    Goat Anti-Feline IgG(H+L)-FITC
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  6. Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-UNLB
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  7. ELISA plate was coated with purified rabbit IgG Fab, IgG Fc, IgG, and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit Light Chain-UNLB (SB Cat. No. 4060-01) followed by Goat Anti-Mouse IgG<sub>2b</sub>, Human ads-HRP (SB Cat. No. 1090-05).
    Mouse Anti-Rabbit Light Chain-UNLB (SB62a)
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  8. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  9. ELISA plate was coated with purified rabbit IgG and IgM and mouse, rat, human, goat, hamster, bovine, horse, sheep, chicken, and guinea pig serum. Immunoglobulins and sera were detected with Swine Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB (SB Cat. No. 6312-01; 1 mg/mL) followed by Goat Anti-Porcine IgG(H+L)-HRP (SB Cat. No. 6050-05).
    Swine Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB
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  10. ELISA plate was coated with purified rabbit IgM and IgG. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgM-UNLB (SB Cat. No. 4020-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rabbit IgM-UNLB
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  11. ELISA plate was coated with purified rabbit IgG and IgM and human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit Ig, Human ads-UNLB (SB Cat. No. 4010-01) followed by Swine Anti-Goat IgG(H+L), Human/Rat/ Mouse SP ads-HRP (SB Cat. No. 6300-05).
    Goat Anti-Rabbit Ig, Human ads-UNLB
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  12. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L)-UNLB (SB Cat. No. 6441-01) followed by Goat Anti-Equine IgG(H+L)-HRP (SB Cat. No. 6040-05).
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  13. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  14. ELISA plate was coated with purified rabbit IgG and IgM and mouse, human, goat, and sheep serum. Immunoglobulins and sera were detected with serially diluted Mouse Anti-Rabbit IgG(H+L), Mouse/Human/Goat/Sheep SP ads-UNLB (SB Cat. No. 4091-01) followed by Goat Anti-Mouse IgG, Human ads-HRP (SB Cat. No. 1030-05).
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  15. ELISA plate was coated with purified rabbit IgG Fc, IgG Fab, IgG, and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-UNLB (SB Cat. No. 4041-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
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  16. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with Swine Anti-Rabbit IgG(H+L)-UNLB (SB Cat. No. 6311-01) followed by Goat Anti-Porcine IgG(H+L)-HRP (SB Cat. No. 6050-05).
    Swine Anti-Rabbit IgG(H+L)-UNLB
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  17. Goat Anti-Rabbit IgM, Multi-Species SP ads-UNLB
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  18. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-UNLB (SB Cat. No. 4030-01) followed by Mouse Anti-Goat IgG Fc-HRP (SB Cat. No. 6158-05).
    Goat Anti-Rabbit IgG-UNLB
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  19. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-UNLB (SB Cat. No. 6440-01) followed by Goat Anti-Equine IgG(H+L)-HRP (SB Cat. No. 6040-05).
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  25. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-UNLB (SB Cat. No. 4090-01) followed by Goat Anti-Mouse IgG<sub>1</sub>, Human ads-HRP (SB Cat. No. 1070-05).
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  26. ELISA plate was coated with purified rabbit IgG and IgM and human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum. Immunoglobulins and sera were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Multi-Species SP ads-UNLB (SB Cat. No. 6442-01) followed by Goat Anti-Equine IgG(H+L)-HRP (SB Cat. No. 6040-05).
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  27. Goat Anti-Rabbit IgG Fab, Mouse/Human SP ads-UNLB
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  28. ELISA plate was coated with purified rabbit IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fab-UNLB (SB Cat. No. 4040-01) followed by Rabbit Anti-Goat IgG(H+L)-HRP (SB Cat. No. 6160-05).
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  29. ELISA plate was coated with serially diluted Rabbit F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0112-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG F(ab')<sub>2</sub>-BIOT and Goat Anti-Rabbit IgG Fc-BIOT (SB Cat. No. 4041-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Rabbit F(ab')2 IgG-UNLB
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