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  • Donkey F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-HRP

Donkey F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-HRP

Cat. No.:
6446-05
Donkey F(ab')2 Anti-Rabbit IgG(H+L)-HRP antibody with minimal reactivity to human, mouse, and rat serum proteins for use in ELISA assays.
$143.00
Size Price (USD) Quantity
1.0 mL $143.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM
Source Pepsin digest of Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads (SB Cat. No. 6440)
Cross Adsorption Mouse, rat, and human serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Conjugate HRP (Horseradish Peroxidase)
Buffer Formulation 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Clonality Polyclonal
Concentration Lot specific
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796392

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. RNA helicase A (RHA) positive and normal human serum was resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-RHA followed by Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4052-05) and chemiluminescent detection.<br/>Image from Vázquez-Del Mercado M, Palafox-Sánchez CA, Muñoz-Valle JF, Orozco-Barocio G, Oregon-Romero E, Navarro-Hernández RE, et al. High prevalence of autoantibodies to RNA helicase A in Mexican patients with systemic lupus erythematosus. Arthritis Res Ther. 2010;12:R6. Figure 1(b)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/2.0/
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  2. Normal Donkey Serum
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  3. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Multi-Species SP ads-HRP (SB Cat. No. 6444-05).
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  4. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat F(ab')<sub>2</sub> Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-HRP (SB Cat. No. 4054-05).
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  5. Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rabbit Anti-Human DR5-UNLB (SB Cat. No. 6600-01). Proteins were visualized using Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP (SB Cat. No. 4050-05) secondary antibody and chemiluminescent detection.
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  6. Reduced (Lane 1) and non-reduced rabbit IgG (Lane 2) was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit Light Chain-HRP (SB Cat. No. 4060-05). Light chains were visualized using chemiluminescent detection.
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  7. Rabbit IgG-UNLB (SB Cat. No. 0111-01) was resolved by electrophoresis, transferred to PVDF membrane, and visualized using Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05) secondary antibody and chemiluminescent detection.
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  8. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with Swine Anti-Rabbit IgG(H+L), Mouse/Rat/Human/Goat SP ads-HRP (SB Cat. No. 6310-05).
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  10. Lysates from mouse embryonic fibroblasts expressing no Bak (Bax-/-Bak-/- (DKO)), mouse Bak (Bax-/-), or WT human Bak (in DKO) were resolved by electrophoresis, transferred to nitrocellulose membrane, and probed with anti-Bak followed by Goat Anti-Rabbit Ig, Human ads-HRP (SB Cat. No. 4010-05) and chemiluminescent detection.<br/>Image from Alsop AE, Fennell SC, Bartolo RC, Tan IK, Dewson G, Kluck RM. Dissociation of Bak α1 helix from the core and latch domains is required for apoptosis. Nat Commun. 2015;6:6841. Figure 1(a)
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  11. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  12. ELISA plate was coated with purified rabbit IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG Fc-HRP (SB Cat. No. 4041-05).
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  13. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L)-HRP (SB Cat. No. 6441-05).
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  14. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG-HRP (SB Cat. No. 4030-05).
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  16. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Human ads-HRP (SB Cat. No. 4051-05).
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  17. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-HRP (SB Cat. No. 6440-05).
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  18. ELISA plate was coated with serially diluted Rabbit IgM-UNLB (SB Cat. No. 0113-01). Immunoglobulin was detected with Goat Anti-Rabbit IgG-BIOT (SB Cat. No. 4030-08) and Goat Anti-Rabbit IgM-BIOT (SB Cat. No. 4020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  19. ELISA plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted Goat Anti-Rabbit IgG(H+L), Mouse/Rat/Human ads-HRP (SB Cat. No. 4049-05).
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  20. Lane 1 - Rabbit IgG<br/>Lane 2 - Rabbit IgG Light Chains<br/>Lane 3 - Rabbit IgG Heavy Chains<br/>Rabbit immunoglobulins above were resolved by electrophoresis under reducing conditions, transferred to PVDF membrane, and probed with Mouse Anti-Rabbit IgG-HRP (SB Cat. No. 4090-05). Proteins were visualized using chemiluminescent detection.
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