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Donkey F(ab')2 Anti-Mouse IgG(H+L)-AF488

Cat. No.:
6412-30
Donkey F(ab')2 Anti-Mouse IgG(H+L)-Alexa Fluor® 488 antibody for use in fluorescent assays.
$214.00
Size Price (USD) Quantity
0.5 mg $214.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pepsin digest of Donkey Anti-Mouse IgG(H+L) (SB Cat. No. 6411)
Cross Adsorption None; may react with immunoglobulins from other species
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796321

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  2. Human epithelial carcinoma cell line HEp-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Goat Anti-Mouse IgG(H+L), Human ads-AF488 (SB Cat. No. 1031-30) and DAPI.
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  4. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  5. Human astrocytoma cell line CCF-STTG1 was stained with Mouse Anti-Human GFAP-UNLB (SB Cat. No. 12075-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-FITC (SB Cat. No. 6175-02) and DAPI.
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-AF488 (SB Cat. No. 1038-30) and DAPI.
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  7. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Donkey Anti-Mouse IgG(H+L), Human SP ads-AF488 (SB Cat. No. 6410-30) and DAPI.
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  8. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L)-AF488 (SB Cat. No. 6170-30).
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  10. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Mouse IgG(H+L)-FITC (SB Cat. No. 6120-02).
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  12. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  13. Donkey Anti-Mouse IgG(H+L)-AF488
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  14. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Mouse IgG(H+L), Human ads-FITC (SB Cat. No. 6125-02).
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