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  • Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT

Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT

Cat. No.:
6421-08
Donkey F(ab')2 Anti-Goat IgG(H+L)-Biotin antibody with minimal reactivity to mouse and rat serum proteins for use in ELISA assays.
$134.00
Size Price (USD) Quantity
0.5 mg $134.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey F(ab')2 IgG
Specificity Reacts with the heavy and light chains of goat IgG
Source Pepsin digest of Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads (SB Cat. No. 6420)
Cross Adsorption Mouse and rat serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins
Purification Method Gel filtration chromatography of pepsin digested antibody
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895813

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Rabbit Anti-Goat IgG(H+L), Human SP ads-BIOT (SB Cat. No. 6164-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
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  2. Normal Donkey Serum
    Normal Donkey Serum
    0030-01
    $47.00
  3. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115h)
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  4. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
    Fluoromount-G®
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  5. BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD45R-BIOT (SB Cat. No. 1665-08) followed by Mouse Anti-Biotin-PE (SB Cat. No. 6404-09).
    Mouse Anti-Biotin-PE (SB58c)
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  6. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6158-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115d)
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  7. ELISA plate was coated with purified goat IgG Fab and IgG Fc. Immunoglobulins were detected with serially diluted Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Rabbit Anti-Goat IgG Fab-BIOT
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  8. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
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  9. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
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  10. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
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  11. Paraffin embedded rat kidney section post 30% blood volume loss and treatment with ketoprofen was stained with anti-IL-1 followed by Rabbit Anti-Goat IgG(H+L)-BIOT (SB Cat. No. 6160-08), HRP conjugated streptavidin, DAB, and hematoxylin.<br>Image from Guedes FS Jr, Cruz DS, Rodrigues MM, Silva LM, Amorim RL, Vianna PT, et al. Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect. Acta Cir Bras. 2012;27:37-42. Figure 3
    Rabbit Anti-Goat IgG(H+L)-BIOT
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  12. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (
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  13. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  14. ELISA plate was coated with serially diluted Goat IgG Fab-UNLB (SB Cat. No. 0131-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fc-BIOT and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (SB Cat No. 7100-05)
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