Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT
Cat. No.:
6430-08
Donkey Anti-Rat IgG(H+L)-Biotin antibody with minimal reactivity to mouse serum proteins for use in ELISA, immunohistochemistry / immunocytochemistry, and western blot assays.
$116.00
![NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.](https://www.southernbiotech.com/media/catalog/product/cache/7149a3d30fe6da616557c86e375dcb18/8/6/864c264e4ec32bdd857d07f4cb1af3f1d9839b89a12dd36acc6b1b8f9a76ed2e.jpeg)
![NIH/Swiss mouse fibroblast cell line 3T3 was stained with Rat Anti-β-Actin-UNLB (right) followed by Donkey Anti-Rat IgG(H+L), Mouse SP ads-BIOT (SB Cat. No. 6430-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.](https://www.southernbiotech.com/media/catalog/product/cache/17c388778a1e936a941c20f268c3f870/8/6/864c264e4ec32bdd857d07f4cb1af3f1d9839b89a12dd36acc6b1b8f9a76ed2e.jpeg)
Isotype | Donkey IgG |
---|---|
Isotype Control | 0175-08 |
Specificity | Reacts with the heavy and light chains of rat IgG and the light chains of rat IgM |
Source | Pooled antisera from donkeys hyperimmunized with rat IgG |
Cross Adsorption | Mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other rat immunoglobulins |
Purification Method | Affinity chromatography on rat IgG covalently linked to agarose |
Conjugate | BIOT (Biotin) |
Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
Clonality | Polyclonal |
Concentration | 0.5 mg/mL |
Volume | 2.0 mL |
Recommended Storage | 2-8°C |
Applications |
Quality tested applications for relevant formats include - ELISA FLISA Other referenced applications for relevant formats include - Immunohistochemistry-Paraffin Sections 1 Immunocytochemistry 3 Western Blot 2 |
RRID Number | AB_2796355 |
Documentation
Certificate of Analysis Lookup
Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view
- 1. Basso K, Schneider C, Shen Q, Holmes AB, Setty M, Leslie C, et al. BCL6 positively regulates AID and germinal center gene expression via repression of miR-155. J Exp Med. 2012;209:2455-65. (IHC-PS)
- 2. SouthernBiotech unpublished data (WB)
- 3. SouthernBiotech published data (ICC)