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  • Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-CY5

Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-CY5

Cat. No.:
6415-15
Donkey Anti-Mouse IgG(H+L)-CY5 antibody with minimal reactivity to human, goat, sheep, bovine, rabbit, chicken, horse, and guinea pig serum proteins for use in immunocytochemistry assays.
$203.00
Size Price (USD) Quantity
1.0 mg $203.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-15
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from donkeys hyperimmunized with mouse IgG
Cross Adsorption Goat, sheep, bovine, human, rabbit, chicken, horse, and guinea pig serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate CY5 (Cyanine 5)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Cy® is a registered trademark of Global Life Sciences Solutions Germany GmbH
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

Other referenced applications for relevant formats include -
Immunocytochemistry 1
RRID Number AB_2895811

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. SouthernBiotech published data (ICC)

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  1. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Donkey Anti-Mouse IgG(H+L), Human SP ads-CY5 (SB Cat. No. 6410-15).
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  2. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Human ads-CY5 (SB Cat. No. 1031-15).
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  5. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Human ads-AF647 (SB Cat. No. 1031-31).
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  6. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  7. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-AF647 (SB Cat. No. 1038-31).
    Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-AF647
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  8. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L)-AF647 (SB Cat. No. 6170-31).
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  9. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  11. FLISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L)-AF647 (SB Cat. No. 1036-31).
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