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Donkey Anti-Mouse IgG(H+L), Human SP ads-BIOT

Cat. No.:
6410-08
Donkey Anti-Mouse IgG(H+L)-Biotin antibody with minimal reactivity to human serum proteins for use in ELISA, flow cytometry, and immunohistochemistry / immunocytochemistry assays.
$116.00
Size Price (USD) Quantity
1.0 mg $116.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-08
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from donkeys hyperimmunized with mouse IgG
Cross Adsorption Human serum proteins (SP); may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA 1,2
FLISA

Other referenced applications for relevant formats include -
Flow Cytometry 5
Immunohistochemistry-Frozen Sections 3,4
Immunocytochemistry 6
RRID Number AB_2796305

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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Other Conjugates and Formulations

Citations

  1. 1. Graham KL, Thibault DL, Steinman JB, Okeke L, Kao PN, Utz PJ. Granzyme B is dispensable for immunologic tolerance to self in a murine model of systemic lupus erythematosus. Arthritis Rheum. 2005;52:1684-93. (ELISA)
  2. 2. Parker MJ, Xue S, Alexander JJ, Wasserfall CH, Campbell-Thompson ML, Battaglia M, et al. Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice. Diabetes. 2009;58:2277-84. (ELISA)
  3. 3. Broekema M, Harmsen MC, van Luyn MJ, Koerts JA, Petersen AH, van Kooten TG, et al. Bone marrow-derived myofibroblasts contribute to the renal interstitial myofibroblast population and produce procollagen I after ischemia/reperfusion in rats. J Am Soc Nephrol. 2007;18:165-75. (IHC-FS)
  4. 4. Li Y, Qin X, Kuang F, Wu R, Duan X, Ju G, et al. Alterations of Fc gamma receptor I and Toll-like receptor 4 mediate the antiinflammatory actions of microglia and astrocytes after adrenaline-induced blood-brain barrier opening in rats. J Neurosci Res. 2008;86:3556-65. (IHC-FS)
  5. 5. SouthernBiotech unpublished data (FC)
  6. 6. SouthernBiotech published data (ICC)
See All References

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  4. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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