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Donkey Anti-Mouse IgG(H+L)-BIOT

Cat. No.:
6411-08
Donkey Anti-Mouse IgG(H+L)-Biotin antibody for use in ELISA assays.
$94.00
Size Price (USD) Quantity
1.0 mg $94.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-08
Specificity Reacts with the heavy and light chains of mouse IgG and the light chains of mouse IgM and IgA
Source Pooled antisera from donkeys hyperimmunized with mouse IgG
Cross Adsorption None; may react with immunoglobulins from other species
Purification Method Affinity chromatography on mouse IgG covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 2.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2796313

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Donkey Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6415-08), Streptavidin-TXRD (SB Cat. No. 7100-07), and DAPI.
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  3. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 1031-08) followed by Streptavidin-FITC (SB Cat. No. 7100-02).
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  4. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  5. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Rabbit Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6175-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  6. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 1038-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  7. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
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  8. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Human CD44-UNLB (SB Cat. No. 9400-01; right) followed by Donkey Anti-Mouse IgG(H+L), Human SP ads-BIOT (SB Cat. No. 6410-08), Streptavidin-TRITC (SB Cat. No. 7100-03), and DAPI.
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  9. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with Rabbit Anti-Mouse IgG(H+L)-BIOT (SB Cat. No. 6170-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  11. Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse IgG(H+L), Human/Bovine/Horse SP ads-BIOT (SB Cat. No. 1037-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
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  12. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Rabbit F(ab')<sub>2</sub> Anti-Mouse IgG(H+L)-BIOT (SB Cat. No. 6120-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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  13. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Goat Anti-Mouse IgG(H+L), Rat ads-BIOT (SB Cat. No. 1034-08) and Streptavidin-FITC (SB Cat. No. 7100-02).
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  14. ELISA plate was coated with serially diluted Mouse IgG-UNLB (SB Cat. No. 0107-01). Immunoglobulin was detected with Goat Anti-Mouse IgG, Human ads-BIOT (SB Cat. No. 1030-08), Goat Anti-Mouse IgA-BIOT (SB Cat. No. 1040-08), and Goat Anti-Mouse IgM, Human ads-BIOT (SB Cat. No. 1020-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
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  17. Frozen newborn mouse cartilage sections were blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Mouse Anti-Collagen Type II-UNLB (right) followed by Rabbit F(ab')<sub>2</sub> Anti-Mouse IgG(H+L), Human ads-BIOT (SB Cat. No. 6125-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
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  18. ELISA plate was coated with purified mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Mouse IgG(H+L)-BIOT (SB Cat. No. 1036-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
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