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  • Donkey Anti-Goat IgG Fc-BIOT

Donkey Anti-Goat IgG Fc-BIOT

Cat. No.:
6460-08
Donkey Anti-Goat IgG Fc-Biotin antibody for use in ELISA assays.
$99.00
Size Price (USD) Quantity
0.5 mg $99.00
Technical Bulletin Safety Datasheet

Product Specifications

More Information
Isotype Donkey IgG
Isotype Control 0175-08
Specificity Reacts with the Fc region of goat IgG
Source Pooled antisera from donkeys hyperimmunized with goat IgG Fc
Cross Adsorption Goat IgG Fab; may react with immunoglobulins from other species
Purification Method Affinity chromatography on goat IgG Fc covalently linked to agarose
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Quality tested applications for relevant formats include -
ELISA
FLISA

RRID Number AB_2895834

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view


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  1. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6158-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115d)
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  2. ELISA plate was coated with Goat Anti-Human IgG-BIOT (SB Cat. No. 2040-08) and purified/unlabeled Rat IgG<sub>1</sub>κ. Biotin conjugated antibody and purified immunoglobulin were detected with serially diluted Streptavidin-HRP (SB Cat. No. 7105-05).
    Streptavidin-HRP
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  3. ELISA plate was coated with purified goat IgG Fc and IgG Fab. Immunoglobulins were detected with serially diluted Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat. No. 7100-05).
    Mouse Anti-Goat IgG Fc-BIOT (SB115h)
    6157-08
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  4. Paraffin embedded rat kidney section post 30% blood volume loss and treatment with ketoprofen was stained with anti-IL-1 followed by Rabbit Anti-Goat IgG(H+L)-BIOT (SB Cat. No. 6160-08), HRP conjugated streptavidin, DAB, and hematoxylin.<br>Image from Guedes FS Jr, Cruz DS, Rodrigues MM, Silva LM, Amorim RL, Vianna PT, et al. Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect. Acta Cir Bras. 2012;27:37-42. Figure 3
    Rabbit Anti-Goat IgG(H+L)-BIOT
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  5. Paraffin embedded mouse small intestine tissue section was blocked with Normal Rabbit Serum (SB Cat. No. 0040-01) and stained with Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01) followed by Rabbit Anti-Goat IgG(H+L), Human SP ads-BIOT (SB Cat. No. 6164-08), Streptavidin-FITC (SB Cat. No. 7100-02), DAPI, and mounted with Fluoromount-G<sup>&reg;</sup> Anti-Fade (SB Cat. No. 0100-35).
    Rabbit Anti-Goat IgG(H+L), Human SP ads-BIOT
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  6. ELISA plate was coated with serially diluted Goat IgG Fc-UNLB (SB Cat. No. 0130-01). Immunoglobulin was detected with Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) and Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) followed by Streptavidin-HRP (
    Goat IgG Fc-UNLB
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  7. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.
    Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT
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  8. Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.
    Goat IgG-UNLB
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  9. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT (SB Cat. No. 6420-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT
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  10. ELISA plate was coated with serially diluted Goat F(ab')<sub>2</sub> IgG-UNLB (SB Cat. No. 0110-01). Immunoglobulin was detected with Rabbit Anti-Goat IgG Fab-BIOT (SB Cat. No. 6022-08) and Mouse Anti-Goat IgG Fc-BIOT (SB Cat. No. 6157-08) followed by Streptavidin-HRP (SB Cat No. 7100-05) and quantified.
    Goat F(ab')2 IgG-UNLB
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  11. Frozen newborn mouse cartilage section was stained with Goat Anti-Type II Collagen-UNLB (SB Cat. No. 1320-01; right) followed by Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT (SB Cat. No. 6425-08), Streptavidin-FITC (SB Cat. No. 7100-02), and DAPI.
    Donkey Anti-Goat IgG(H+L), Multi-Species SP ads-BIOT
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  12. Paraffin embedded sections of mouse P8 testes were stained with anti-BCAS2 and anti-PLZF. The DNA was stained with Hoechst 33342 and mounted with Fluoromount-G<sup>&reg;</sup> (SB Cat. No. 0100-01).<br/>Images from Liu W, Wang F, Xu Q, Shi J, Zhang X, Liu X, et al. BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis. Nat Commun. 2017;8:14182. Figure 1(d)<br/>Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
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  13. ELISA plate was coated with purified goat IgG. Immunoglobulin was detected with Donkey F(ab')<sub>2</sub> Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT (SB Cat. No. 6421-08) followed by Streptavidin-HRP (SB Cat. No. 7105-05).
    Donkey F(ab')2 Anti-Goat IgG(H+L), Mouse/Rat SP ads-BIOT
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