Custom Monoclonal Antibody Production

Take advantage of SouthernBiotech’s 25+ years of experience providing comprehensive custom monoclonal antibody production services and choose our expert team for your next project. We produce monoclonal antibodies at all scales, from small batches to large lots, driving successful research in academia, biopharma, and the diagnostics industries.

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Service Overview

With a focus on quality and customer service, SouthernBiotech’s custom monoclonal antibody production team is ready to generate these critical reagents to advance your project. Every service is customized to meet your needs and can include any or all of the following activities:

  • Peptide antigen design, synthesis, and carrier protein conjugation
  • Animal immunization in mouse or rat
  • Fusion for hybridoma creation
  • Positive and negative screening
  • Subcloning
  • Expansion
  • Cryopreservation
  • Final antibody purification
  • Antibody conjugation
  • Antibody characterization and testing

For example, we can perform hybridoma generation only, or hybridoma generation plus purification and more. We are also able to amplify and purify existing client-supplied cell lines, perform fusions of client-immunized splenocytes, and purify client-supplied supernatant and ascites.

Whatever services you choose, you can be confident in the quality with every step of the production process conducted in-house in our ISO 9001:2015 certified facilities.

We also ensure you stay up-to-date on the status of your service through a dedicated project manager who coordinates regular communications and is available to answer any questions.

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Expert technical support

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Customizable to suit your needs

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100% in-house production

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Custom Monoclonal Antibody Production Process

Each phase is optional and can be combined in any number of ways.

Antigen Design and Preparation

We accept various antigen types including peptides, recombinant proteins, and purified native proteins. These may be supplied in a variety of different formats such as in solution in a neutral buffer, a soluble lyophilisate, and contained in gel slices or on nitrocellulose blots.

Available Antigen Services

  • Consultation regarding peptide design
  • Peptide synthesis services
  • Peptide conjugation to KLH for immunization
  • Peptide conjugation to BSA for purification and/or screening
  • Homogenization from slices

Immunization and ELISA Titration

Animals are immunized and boosted over several weeks. We select the best-responding animal using a titration ELISA and send you the results. Alternatively, you may opt to have the sera shipped to you to conduct the evaluation in your own lab.

Fusion and Screening for Positive Supernatants

The best-responding animal(s) will receive additional boosts, after which we harvest splenocytes for fusion with a selected myeloma cell line. We then screen for positive supernatants using ELISA and provide you with the results. We can also ship you the supernatant from the best hybridoma-containing wells for evaluation in your lab.

Subcloning, Expansion, and Cryopreservation

We obtain monoclonal cell lines by performing limiting dilution of the hybridoma(s). This also helps ensure the long-term stability of the cells. Once monoclonality is achieved, we confirm the isotype and screen for positive clones using ELISA. We send you the ELISA results and/or we can ship the supernatant from the most promising wells to your lab for additional evaluation. The selected clones are then expanded and cyropreserved. We can also provide cell storage services.

Amplification

We can produce selected clones in vitro (cell culture flask or bioreactor supernatant) or in vivo (mouse ascites with an immunocompromised option). Small or large scale production is available.

Antibody Purification

Supernatant or ascites may be shipped separately, pooled following the final collections, or pooled and purified utilizing a selection of the following methods:

  • Protein G purification
  • Protein A purification
  • Ammonium sulfate precipitation
  • Caprylic acid precipitation
  • PEG precipitation
  • Affinity purification
  • Size exclusion / Gel filtration chromatography
  • Endotoxin removal

Antibody Conjugation

SouthernBiotech offers a variety of conjugation options, to support your different assay needs, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), biotin, β-galactosidase and fluorochromes including Alexa Fluor® dyes, FITC, PE, and PE/CY7.

Antibody Testing and Characterization

We can characterize your antibody using any of the following:

  • Serum titer evaluation
  • ELISA testing for specific activity
  • Endotoxin testing by LAL assay
  • HPLC
  • Western blot
  • Flow cytometry
  • Immunohistochemistry

Initiate a Custom Service Project

Proven expertise, outstanding service, and responsive customer support are just a few of the reasons why scientists continue to turn to SouthernBiotech for custom monoclonal antibody production services.

Start an antibody production project with us and experience the SouthernBiotech difference for yourself.

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