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Mouse Anti-Human IgD-AF488 (IADB6)
Cat. No.:
9030-30
Alexa Fluor® 488 Anti-Human IgD antibody for use in flow cytometry and immunohistochemistry assays.
$289.00
| Clone | IADB6 |
|---|---|
| Isotype | Mouse IgG2aκ |
| Isotype Control | Mouse IgG2a-AF488 (HOPC-1) |
| Specificity | Human IgD |
| Immunogen | Human IgD myeloma protein |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | ELISA – Quality tested FLISA – Quality tested Flow Cytometry – Quality tested 4-10 Immunohistochemistry-Frozen Sections – Reported in literature 2 Immunohistochemistry-Paraffin Sections – Reported in literature 3 Immunoprecipitation – Reported in literature 1 Separation – Reported in literature 11,12 Costimulation – Reported in literature 13,14 |
| RRID Number | AB_2796596 |
| Gene ID | 3495 (Human) |
| Gene ID Symbol | IGHD (Human) |
| UniProt ID | P01880 (Human |
| UniProt Name | IGHD_HUMAN (Human) |
Documentation
Certificate of Analysis Lookup
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- 1. Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H. Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987;138:4150-8. (IP)
- 2. Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, et al. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells. J Immunol. 2007;178:2872-82. (IHC-FS)
- 3. Shi S, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 1991;39:741-8. (IHC-PS)
- 4. Klein U, Küppers R, Rajewsky K. Variable region gene analysis of B cell subsets derived from a 4-year-old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age. J Exp Med. 1994;180:1383-93. (FC)
- 5. Krzysiek R, Lefèvre EA, Zou W, Foussat A, Bernard J, Portier A, et al. Antigen receptor engagement selectively induces macrophage inflammatory protein-1α (MIP-1α) and MIP-1β chemokine production in human B cells. J Immunol. 1999;162:4455-63. (FC)
- 6. Jaimes MC, Rojas OL, Kunkel EJ, Lazarus NH, Soler D, Butcher EC, et al. Maturation and trafficking markers on rotavirus-specific B cells during acute infection and convalescence in children. J Virol. 2004;78:10967-76. (FC)
- 7. He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, et al. Intestinal bacteria trigger T cell-independent immunoglobulin A2 class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity. 2007;26:812-26. (FC)
- 8. Rojas OL, Caicedo L, Guzmán C, Rodríguez L, Castañeda J, Uribe L, et al. Evaluation of circulating intestinally committed memory B cells in children vaccinated with attenuated human rotavirus vaccine. Viral Immunol. 2007;20:300-11. (FC)
- 9. Smith K, Garman L, Wrammert J, Zheng N, Capra JD, Ahmed R, et al. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nature Protoc. 2009;4:372-84. (FC)
- 10. Suryani S, Fulcher DA, Santner-Nanan B, Nanan R, Wong M, Shaw PJ, et al. Differential expression of CD21 identifies developmentally and functionally distinct subsets of human transitional B cells. Blood. 2010;115:519-29. (FC)
- 11. Al-Darmaki S, Knightshead K, Ishihara Y, Best A, Schenkein HA, Tew JG, et al. Delineation of the role of platelet-activating factor in the immunoglobulin G2 antibody response. Clin Diagn Lab Immunol. 2004;11:720-8. (Sep)
- 12. Xu W, Santini PA, Matthews AJ, Chiu A, Plebani A, He B, et al. Viral double-stranded RNA triggers Ig class switching by activating upper respiratory mucosa B cells through an innate TLR3 pathway involving BAFF. J Immunol. 2008;181:276-87. (Sep)
- 13. Kuritani T, Cooper MD. Human B cell differentiation. III. Enhancing effect of monoclonal anti-immunoglobulin D antibody on pokeweed mitogen-induced plasma cell differentiation. J Immunol. 1982;129:2490-5. (Costim)
- 14. Igarashi H, Sakaguchi N. Telomerase activity is induced in human peripheral B lymphocytes by the stimulation to antigen receptor. Blood. 1997;89:1299-1307. (Costim)
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