Mouse anti-Human Interleukin-21 (IL-21)-ELISA Set
Interleukin-21 (IL-21) is a four helical bundle type I cytokine
. The mature form of human IL-21 is a 131 amino acid protein.
IL-21 is expressed primarily by NKT and CD4+ T cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses. The effects of IL-21 include promotion of antibody production, NK cell differentiation, proliferation and differentiation of cells of monocyte and granulocyte lineages, costimulation of T cells and augmentation of antitumor activity of CD8+ T cells.
Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases. Principle of Assay This assay incorporates a quantitative sandwich enzyme immunoassay technique. Unlabeled monoclonal antibody specific for human IL-21 is first coated onto 96 well microplate(s). Standards and samples are then added to the wells and any IL-21 present is captured by the immobilized antibody. After a wash to remove unbound material, biotin-labeled anti-human IL-21 detection antibody is added to the wells, followed by a horseradish peroxidase-labeled anti-biotin antibody incubation. After another wash, TMB substrate solution is added that will result in a blue color proportional to the amount of bound IL-21. Color development is then quenched and intensity is measured at 450nm.
[Technical Bulletin / References ]
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Anti-Human IL-21 ELISA Set
- purified, unlabeled antibody
- fluorescein isothiocyanate-labeled
- tetramethlyrhodamine isothiocyanate
- alkaline phosphatase-labeled
- horseradish peroxidase-labeled
- Texas Red
- R-phycoerythrin/Texas Red
- low endotoxin/azide-free
(SPRD) is a tandem conjugate of R-phycoerythrin and CY
5. It was developed as a reagent for 3-color flow cytometry on instruments with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.
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