ApoScreen Annexin V
|
|
ANTIGEN DESCRIPTION
|
|
|
|
|
|
SouthernBiotech Cat. No. 10039 is a recombinant protein that is produced in eukaryotic cells.
A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface.[1] Annexin V (Mr 36-kDa), a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers.[2,3]. When PS is exposed on the extracellular face of a cell membrane, Annexin V binds with an affinity of approximately 7 nM[4]. Fluorochrome-conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry,[5-8] thereby providing a convenient tool for detection of apoptotic cells.
Annexin V-FITC is a labeled Annexin V that can be used to evaluate subpopulations of cells along the apoptotic pathway without counterstaining with propidium iodide or other DNA dyes. Analysis is performed on a flow cytometer equipped with a 488 nm argon laser and gated on viable cells by light scatter (see flow histograms below). Alternatively, the cells can be examined by fluorescence microscopy.
|
|
|
|
|
| Cat. No. | Product Description | Clone | Size |
USD Price
|
Order Qty
|
10039-01
|
Aposcreen Annexin V-UNLB
|
|
100 Tests
|
$185.00
|
|
10039-02
|
Aposcreen Annexin V-FITC
|
|
100 tests
|
$195.00
|
|
10039-08
|
Aposcreen Annexin V-BIOT
|
|
0.1 mg
|
$280.00
|
|
|
FORMAT ABBREVIATIONS:
|
UNLB - purified, unlabeled antibody
FITC - fluorescein isothiocyanate-labeled
TRITC - tetramethlyrhodamine isothiocyanate
AP - alkaline phosphatase-labeled
HRP - horseradish peroxidase-labeled
BGAL - B-galactosidase-labeled
TXRD - Texas RedTM
BIOT - biotin-labeled
R-PE - R-phycoerythrin-labeled
R-PE/TXRD - R-phycoerythrin/Texas RedTM
APC - allophycocyanin
|
CY3 - CyanineTM 3-labeled *
SPRD - SpectralRedTM-labeled *
LE/AF - low endotoxin/azide-free
CY5 - CyanineTM5-labeled *
R-PE/CY5.5 - R-phycoerythrin/CyanineTM5.5-labeled *
R-PE/CY7 - R-phycoerythrin/CyanineTM7-labeled *
APC/CY5.5 - allophycocyanin/CyanineTM5.5-labeled *
APC/CY7 - allophycocyanin/CyanineTM7-labeled *
FLMA - fluorescein-5-malemide-labeled
SEPH - sepharose-labeled
BIMA - biotin-maleimide-labeled
|
|
NOTE: *SpectralRedTM (SPRD) is a tandem conjugate of R-phycoerythrin and
CYTM5. It was developed as a reagent for 3-color flow cytometry on instruments
with only 488 nm excitation source. It requires minimal (0-2%) FL2/FL3 compensation.
|
|